地塞米松局部应用对犬完全性脊髓横断伤再生的影响

The effect of administration of DXM to the injured site on the regeneration of spinal cord with complete transection

目的 观察地塞米松局部应用对犬完全性脊髓横断伤再生的影响。方法 将 9条杂种犬分为两组 ,胸10平面造成 4～ 5mm的脊髓缺损 ,实验组 6条犬 ,将饱和状态下地塞米松组织液凝块植入脊髓缺损处。对照组 3条犬 ,仅植入组织液凝块。术后对两组犬的行为学进行评分 ,并用摄像机记录。术后 5个月心脏灌流固定 ,石腊包埋 ,切片。选用神经中丝抗体 (anti-neurofiblament ,NF)对桥接区组织内神经轴突进行标记 ,计算两组NF的阳性表达率。 6号犬和 9号犬桥接区组织同时行电镜观察。结果 实验组行为学评分明显高于对照组 ,有显著性差异 ( 0 .0 1<P <0 .0 5 )。实验组桥接区NF阳性表达率 1%～ 5 % (平均 2 .86% ) ,对照组桥接区NF的阳性表达为 0 %。

Objective To investigate the effect of administration of DXM(dexamethasome) to the injured site on the regeneration of spinal cord with complete transection. Methods Nine crossbreed canines were diveided into two groups, and at T 10 level of their spinal cords 4～5 mm default was made. Six canines were in the experimental group, and tissue liquid clot saturated with DXM was transplanted into the default. Three canines were in the control group, only tissue liquid clot was transplanted into the default. After operation the motor function of hind limbs of canined in two groups was evaluated and photographed. Five months after operation the hearts of the canines were affused to fix the bridged area and sample was taken, embed in olefin and sliced up. In the bridged area anti-NF immunohistochemistry was used to label the axons, and NF positive expression rate was calculated. The bridged area of number six and nine canine was observed with electron microscope. Results The grade of motor function of hind limbs of canines in experimental group is apparently higher than the control group, and there is significance difference (0.01< P <0.05). NF positive expression rate in the experimental group is 1%～5% (averaged 2.86%), and NF positive expression rate in the control group was 0%. Conclusion The administration of DXM to the injured site of canine can improve the regeneration of spinal cord with complete transection.