HPLC测定天麻钩藤颗粒中黄芩苷的含量

Determination of baicalin in Tianma Gouteng granules by HPLC

目的 采用HPLC测定天麻钩藤颗粒中黄芩苷的含量。方法 采用LunaC18(2 )柱 (15 0mm× 4 .6mm ,ID) ,以乙腈 -水-磷酸 (2 0∶80∶0 .1)为流动相 ,检测波长 2 80nm。结果 黄芩苷和制剂中的其他组分可以完全分离。黄芩苷进样量在 0 .10～2 .0 μg范围内 ,峰面积与进样量线性关系良好 (r=0 .9999)。方法回收率为 10 0 .4 %。 结论 本法准确、简便 。

OBJECTIVE An HPLC method was developed for determination of baicalin in Tianma Gouteng granules. METHODS Chromatographic conditions include an ODS column and the mobile phase consisting of acetonitrile-water-phosphoric acid (20∶80∶0.1). The detecting wavelength was set at 280 nm.RESULTS The linear range of the standard curve was 0.10-2.0 μg( r =0.9999), and the average recovery was 100.4%. CONCLUSION The method is simple and accurate. It can be used for the determination of baicalin in Tianma Gouteng granules.