荧光原位杂交技术检测性染色体在异基因干细胞移植中的应用

Application of two-color interphase FISH using sex probe in allogeneic stem cell transplantation

目的 探讨性染色体双色间期荧光原位杂交 (FISH )技术在异性间异基因造血干细胞移植后植活标志、微小残留病灶 (MRD)监测的价值。方法 用间期FISH检测 10例白血病、2例地中海贫血患者异性间移植后不同时期性染色体荧光杂交信号 ,确定供体源的植入克隆或患者的残存克隆。结果 12例患者在移植后 2 2～ 3 5天 ,细胞遗传学及双色间期FISH分析均获植入证据。在移植后不同时间 ,当染色体分析 10 0 %XX或 10 0 %XY结果 ,同步的bcr/ablmRNA基因持续阴性时 ,FISH可显示不同比例的供者源性染色体。结论 双色FISH应用于异性间异基因造血干细胞移植后植活标志、MRD监测 ,操作简易快速 ,是细胞遗传学及分子学检查很好的补充。

Objective To evaluate the significance of two color interphase fluorescence in situ hybridization(FISH )using X and Y centromere probe in the engraftment estimation and minimal residual disease(MRD) monitoring after allogeneic stem cell transplantation(alloSCT).Methods Samples from 12 cases patients in different periods after alloSCT were detected by interphase FISH.Results All of 12 patients had been determined to obtain engraftment in 22～35 day after alloSCT.When traditional karyotypes showed as 100%XX or 100%XY invariably,while bcr/abl mRNA gene express continuously negative,FISH showed different percentage of donor original sex chromosome.Conclusion Two color interphase FISH is more reliably sensitive and simple test for engraftment evaluation and MRD monitoring post SCT,it can be good complement of traditional karyotype analysis and gene detection by RT PCR.