盐酸氨溴索对大鼠肺间质纤维化的作用

The effect of ambroxol on pulmonary interstitial fibrosis in rats and nuckear factor-κB

目的 观察核因子 κB(NF κB)活性在博来霉素 (BLM )所致大鼠肺间质纤维化 (IPF)过程中的动态变化。探讨盐酸氨溴索对大鼠肺间质纤维化的影响及其作用机制。方法 用博来霉素诱导大鼠肺间质纤维化 ,观察各组动物肺脏病理组织学改变 ,通过免疫组织学方法检测各组动物肺脏NF κB、转移生长因子 β1(TGFβ1)、胶原蛋白Ⅰ (ⅢcollagenⅠⅢ )含量。 结果 模型组大鼠肺组织NF κB含量明显增高 ,盐酸氨溴索组大鼠肺组织肺泡炎、肺纤维化程度减轻 ,NF κB、TGFβ1、胶原蛋白ⅠⅢ含量均低于模型组 ,高于对照组。结论 NF κB是肺间质纤维化进展的重要前炎性因子 ,盐酸氨溴索通过下调NF

Objective To observed the dynamic change of the nuckear factor κB(NF κB) activation of the lung in bleomycin induced interstitial pulmonary fibrosis in rats and to investigate the effect of ambroxol on pulmonary fibrosis and the mechanism.Methods Forty five SD rats were divided into threee groups in random:control group,model group and ambroxol treated group.On experimental day(day0),the rats were intratracheally instilled with bleomycin(5 mg/kg body weight) or sterile saline,and then treated with the ambroxol or saline until they were killed.On day 7, 14, 28 after instillation,five rats of each group were sacrificed and the lung were harvested for histopathological examination. The activation (expression) of NF κB,TGFβ 1,collagenⅠ Ⅲ were measured by means of immunohistochemistry technique.Results the activation of NF κB in the lung rised quickly after bleomycin instillatioin and peaked on day 7～14,then declined, the alveolitis and pulmonary fibrosis of ambroxol treated groups were decreased remarkably,the activiation level of NF κB?TGFβ 1?collagenⅠ Ⅲ of treated groups were also decreased than model group.Conclusion NF κB is a key factor in the development of interstitial pulmonary fibrosis and ambroxol can inhibit progress of alveolitis and pulmonary fibrosis.