反相高效液相色谱法测定大黄药材中游离及结合型蒽醌类衍生物的含量

Determination of Aloe-emodin, Rhein, Emodin and Chrysophanol in Radix Rhei by Reversed Phase High Performance Liquid Chromatography

建立了同时测定大黄药材中蒽醌类衍生物含量的RP HPLC法。色谱柱为HypersilC1 8柱 (2 5 0mm×4 6mmi.d .,1 0 μm) ,流动相为甲醇 乙腈 水 (3∶5∶2 ,磷酸调pH 2 .8) ,流速为 1 .0mL min ,柱温为 2 5℃ ,检测波长为 2 2 5nm。在此色谱条件下 ,各组分在 2 0min内均得到良好分离。平均回收率为 98.83 %～ 1 0 0 .9% ;相对标准偏差 0 .68%～ 1 .5 8%。

The aloe-emodin, rhein, emodin and chrysophanol in radix rhei were simultaneously determined by reversed phase-high performance liquid chromatography (RP-HPLC) on a hypersil C-18 column (10 mum, 250 mm x 4.6 mm i.d.), using methol-Acetonitrile-water (30 : 50 : 20 adjusted to pH = 2.8 with phosphoric acid), a flow rate of 1.0 mL/min, the detective wavelength was 225 nm. These 4 components were separated completely within 20 minutes under this chromatographic condition. The average recoveries were from 98.8% to 103.3%, its relative standard deviations were from 0.7% to 1.6%. The method is simple and quick with high accuracy; and it is suitable for the assay of the Aloe-emodin, rhein, emodin and chrysophanol for radix rhei and compound preparations of Radix Rhei.