新的免疫增强剂—草分枝杆菌的实验和临床研究

A New Immunopotentiator:the Experimental and Clinical Studys of Mycobacterialphlei Cell Wall Complex

目的 探讨草分枝杆菌制剂 (乌体林斯 )体外对肿瘤细胞的影响和对机体免疫功能的调理作用。方法 体外实验采用Lovo结肠癌细胞株按不同的浓度加入草分枝杆菌制剂 ,应用流式细胞仪对癌细胞周期动力学进行检测 ,同时观察草分枝杆菌制剂对大肠癌细胞株凋亡和增殖的影响。在临床应用中 ,分实验组和对照组观察肿瘤患者化疗期间应用草分枝杆菌制剂对细胞和体液免疫功能的影响。结果 药物处理后各组S期细胞数率与对照组比较均显著下降 (P <0 .0 1)。实验组与对照组比较 ,细胞增殖指数显著降低 (P <0 .0 1) ;加入草分枝杆菌制剂各实验组与对照组比较细胞凋亡率明显增高 ,有非常显著性差异 (P <0 .0 1)。在临床对比研究中发现肿瘤患者化疗期间应用乌体林斯能够增强细胞免疫功能。结论 草分枝杆菌制剂不仅能直接抑制癌细胞增殖 ,而且能通过调控癌细胞的细胞周期而促进其凋亡。乌体林斯与化疗联合应用可显著减轻化疗对机体免疫功能的抑制 ,增强机体对化疗的耐受性 。

Objective To observe effects of mycobacterium phlei cell wall complex(Utilin′S)on human colorectal cancer cell line(Lovo cells) and on the immunity of patients with colorectal cancers during postoperative chemotherapy.Methods Lovo cells were cultured in RPMI 1640,and with Utilin′S atdifferent concentration of 1.72,0.86,0.43,and 0.215 ng/ml for 48 h,respectively.Cell cycle kinetics,cell apoptosis and cell proliferation were analyzed by flow cytometry.Results The drug had significant effect on Lovo cells cycle rate,the percentage of cells at G 0～G 1 phase increased(P<0.01),while the percentage of cells at S phase decreased(P<0.01).Immune function decreased significantly after chemotherapy in the control group,while in the group treated with Utilin's,cellular immunity increased significantly.Conclusion The drug can directly inhibit tumor proliferation,and may stimulate apoptosis via modulating the tumor cytokinetics.The combination of Utilin S in postoperative chemotherapy can improve the immune function in patients with colorectal cancer.