端粒酶活性及c-myc、p16表达与胃黏膜癌变的相关性研究

Relationship between Telomerase Activity and Expression of c-myc,p16 in Carcinogenesis of Gastric Tumor

目的 研究端粒酶活性及c myc、p16在胃黏膜癌变过程中的作用。 方法 应用PCR -ELISA法检测 171例胃癌及相关组织中端粒酶活性 ;免疫组织化学法检测c myc、p16蛋白表达状况。并分析它们之间的相关性。 结果 胃癌组织端粒酶活性和c myc蛋白的表达率均最高 ,p16蛋白表达率最低 ;慢性浅表性胃炎组织 p16蛋白表达率最高。慢性萎缩性胃炎伴中、重度肠化组织中 ,端粒酶阳性和阴性组c myc和 p16蛋白表达率相互比较均有显著性差异 (P <0 .0 5 ) ;胃癌组织中 ,端粒酶阳性和阴性组c myc蛋白表达率有非常显著性差异 (P <0 .0 1) ;p16的表达率无显著性差异 (P >0 .0 5 )。 结论 在慢性萎缩性胃炎伴中、重度肠化组织中端粒酶活性和c myc的高表达及 p16的失活具有协同性 ,c myc的激活 ,p16的失活可能是端粒酶活化的 1个必要条件。

Objective To study telomerase activity and the expression of c myc,p16 in Carcinogenesis of Gastric mucosa.Methods Telomerase activity of 171 gastric biopsy specimens was detected by PCR ELISA;the expression of c myc and p16 was examined by immunhistochemical technique.Then the correlation of them was analyzed.Results Of all speecmens,telomerase activity and the positive rate of c myc in gastric cancer was the highest( P <0.01),and the expression of p16 was the lowest.In CSG,the expression of p16 was the highest.In CAG with moderate or severe degree IM,the positive rates of c myc、p16 were significantly different between telomerase positive and negative group( P <0.05).In GC,the positive rates of c myc was significantly different between telomerase positive and negative group different( P <0.05),but the positive rates of p16 presented have no significant differences.Conclusion In CAG with moderate or severe degree IM telomerase activity is correlated with high expression of c myc and inactivity of p16.The activation of c myc and inactivation of p16 may be prerequisite to activate the telomerase.