卵巢癌细胞DNA倍体、p21及p53基因表达产物定量分析

The Quantitative Analysis on DNA Ploid and p21,p53 Gene Expression in Ovarian Cancer

目的 探讨卵巢癌DNA倍体、p2 1及 p5 3基因定量表达情况及其临床意义。 方法 应用流式细胞术 (FCM )和荧光免疫技术 ,对 3 9例卵巢癌组织的卵巢癌细胞和 9例正常卵巢组织的卵巢细胞的染色体倍体、p2 1基因和 p5 3基因表达产物进行定量分析。结果 在卵巢癌组织中DNA的异倍体率、p2 1、p5 3基因蛋白产物的阳性表达率均明显高于对照组 ,两者比较均有非常显著性差异 (P <0 .0 1)。结论 应用FCM定量测定卵巢癌细胞中的DNA倍体、p2 1及 p5 3基因表达产物 。

Objective To explore the clinical significance of DNA ploid and p21,p53 gene expression in ovarian cancer.Methods By flow cytometry(FCM) and immunofluorescence staining technique,the DNA ploid,ras p21 and p53 protein expression were quantitatively measured in ovarian cancer cells of 39 cases and in normal ovary cells of 9 healthy people as control.Results The DNA ancuploidy rate,p21 and p53 protein positive expression rate in ovarian cancer was higher than those in control group( P <0.01).Conclusion DNA aneuploid p21 and p53 expression have diagnostic value of ovarian cancer.