增殖细胞核抗原及Bcl-2蛋白在鼻咽癌中的表达

The expression of proliferating cell nuclear antigen and Bcl-2 oncoprotein in nasopharyngeal carcinoma

目的:探讨鼻咽癌组织中增殖细胞核抗原(PCNA)、Bcl-2表达情况及与主要临床指标间的关系。方法:以免疫组化检测鼻咽活检组织中PCNA、Bcl-2蛋白表达,并比较与分析两者和鼻咽癌临床分期及颈淋巴结转移的关系。结果:鼻咽部慢性炎性组织中Bcl-2阳性率为9.5％,明显低于鼻咽癌组织中的阳性率(83.1％)(P<0.01)。PCNA、Bcl-2在鼻咽癌中的表达呈明显负相关(r_s=-0.41,P<0.01)。随着临床分期的增高,PCNA增殖指数(PI)分级有增高的趋势,鼻咽癌伴颈淋巴结转移组中PI之Ⅰ级所占比例,较无颈淋巴结转移组下降,而Ⅱ、Ⅲ、Ⅳ级所占比例增加,但无统计学意义。结论:PCNA PI可反映鼻咽癌的增殖状况,可能与预后相关,Bcl-2蛋白可作为鼻咽癌较好的肿瘤标记物,在鼻咽癌的发生上可能起重要作用。

Objective: To observe PCNA , Bel-2 gene expression and their relationship with clinicopathologic factors in primary nasopharyngeal carcinoma(NPC). Method:PCNANBcl-2 proteins were assayed with immunohis-tochemical technique. Result:The expression of Bcl-2 protein was 9. 5% in chronic inflammation of nasopharyngeal epithelium(CINE) .which was significantly lower than it in NPCs with 83. 1%( P <0. 01). In NPCs, we could find PCNA and Bcl-2 expression were significantly negatively correlated with each other(rs = -0. 41, P <0. 01). In our study,there was some correlation in the grade of PCNA proliferating index(PI) with the clinical stages or cervical lymph node metastasis of NPCs. Conclusion;PCNA PI may represent the proliferative state of NPC,Bcl-2 protein may be a better tumor marker of NPC, all of them may play important roles in the pathogensis of nasopharyngeal carcinoma.