Tiron对抗外源性过氧化氢对离体培养小鼠耳蜗Corti器损伤研究

The protection of Tiron to the injury of organ of Corti cultured in vitro caused by exogenous H_2O_2 in mouse

目的:探讨Tiron能否有效对抗过氧化氢(H_2O_2)对离体培养小鼠Corti器内、外毛细胞的损伤。方法:采用新生小鼠Corti器体外培养技术,建立外源性H_2O_2损伤内外毛细胞模型,给予不同浓度的Tiron,观察对H_2O_2损伤的保护作用、结果:H_2O_2浓度大于1.0mmol/L时,基底膜顶回、中回和底回毛细胞损伤程度不同,底回较重,顶回和中回损伤程度在统计学上无差别。在低于0.5mmol/L时毛细胞损伤与位置无关。在培养液中加入Tiron(10 mmol/L)可以明显减轻H_2O_2对毛细胞的损伤程度。当H_2O_2浓度在0.01～1.0 mmol/L之间时,Tiron几乎可以完全抑制毛细胞的损伤和缺失。结论:Tiron对于小鼠离体培养的Corti器具有明显的保护作用,其对抗H_2O_2损伤的机制可能通过清除超氧阴离子O_2^-和螯合铁离子共同发挥作用。

Objective:In order to observe the action of H2O2 on inner and outer hair cells of organ of Corti in mouse,and discuss whether Tiron can effectively resistent the injury of free radicals in cultured cochlea in vitro or not. Method; Using the culture technology of organ of Corti in neuhorn mouse in vitro, established the model of the injury of inner and outer hair cells caused by exogenous H2O2. And observed the protection to the damage by H2()2 at different concentration of Tiron. Result:When the concentration of H2O2 is higher than 1. 0 mmol/L,the loss of hair cells in the bottom, middle, and parietal turn of basilar membrane vary, the injury of bottom turn is more severe, fn lower than 0. 5 mmol/L group, the injury of hair cells is no relationship with the location. Adding Tiron (10 mmol/L) in culture can apparently decrease the injury of hair cells caused by H2O2. When the concentration of H2O2 is 0. 01~ 1. 0 mmol/L,Tiron can almost inhibit the loss of hair cells completely. Conclusion:There is apparently preventive effection of Tiron on organ of Corti cultured in vitro, and the mechanism of inhibiting injury caused by H2O: is probably by clearing O2 and combining irony ion.