反义端粒酶催化亚单位抑制喉癌细胞生长的离体实验研究

Targeted therapy of human laryngeal squamous cell carcinomain vitro with telomerase catalytic subunit mRNA

目的:探讨人端粒酶催化亚单位(hTERT)反义寡核苷酸对人喉癌细胞株Hep-2的生长抑制作用。方法:用脂质体包裹hTERT反义寡核苷酸转染体外培养的Hep-2细胞,分别于转染后24、48、72h用MTT法检测细胞增殖活性,转染后72h行HE染色及端粒酶活性测定。结果:转染后细胞增殖活性降低;细胞生长密度下降;端粒酶活性受到抑制。结论:hTERT反义寡核苷酸短期内可以降低端粒酶活性、抑制喉癌细胞的生长。

Objective:To study the effect of antisense oligonuclcotide targerted human telomerase catalytic sub-unit (hTERT) on Hep-2 cell line. Method: Cells were treated daily with oligonucleotide (ODN) directed against hTERT,or control ODN-a mismatched sequence or only Lipofectamine. At 24 h, 48 h. 72 h cell viability after administration of ODNs was determined using the MTT assay. At 72 h, the morpholog alteration of the cells was observed by HE stain. At 72 h, telomerase activity was determined. Result; After daily transfection ASODN,some Hep-2 cells begin to stop growing up,but not in the mismatched ODN group or Lipofectamine group. Hep-2 cells treated with antisense against hTERT showed a decrease in telomerase activity. Conclusion: hTERT antisense ODN can inhibit telomerase activity and the growth of Hep-2 cells in short term.