摘要
目的:探讨人端粒酶催化亚单位(hTERT)反义寡核苷酸对人喉癌细胞株Hep-2的生长抑制作用。方法:用脂质体包裹hTERT反义寡核苷酸转染体外培养的Hep-2细胞,分别于转染后24、48、72h用MTT法检测细胞增殖活性,转染后72h行HE染色及端粒酶活性测定。结果:转染后细胞增殖活性降低;细胞生长密度下降;端粒酶活性受到抑制。结论:hTERT反义寡核苷酸短期内可以降低端粒酶活性、抑制喉癌细胞的生长。
Objective:To study the effect of antisense oligonuclcotide targerted human telomerase catalytic sub-unit (hTERT) on Hep-2 cell line. Method: Cells were treated daily with oligonucleotide (ODN) directed against hTERT,or control ODN-a mismatched sequence or only Lipofectamine. At 24 h, 48 h. 72 h cell viability after administration of ODNs was determined using the MTT assay. At 72 h, the morpholog alteration of the cells was observed by HE stain. At 72 h, telomerase activity was determined. Result; After daily transfection ASODN,some Hep-2 cells begin to stop growing up,but not in the mismatched ODN group or Lipofectamine group. Hep-2 cells treated with antisense against hTERT showed a decrease in telomerase activity. Conclusion: hTERT antisense ODN can inhibit telomerase activity and the growth of Hep-2 cells in short term.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2003年第4期226-228,共3页
Journal of Clinical Otorhinolaryngology
基金
湖北省自然科学基金(NO.2001Al4013)
关键词
端粒酶催化亚单位
反义寡核苷酸
喉肿瘤
基因治疗
Human telomerase catalytic subunit
Antisense oligodeoxynucleotide
Laryngeal neoplasms
Genetherapy