人胃癌细胞株抗脱落凋亡特性的分析

Characteristics of anoikis resistance of human gastric cancer cell lines

目的 :研究胃癌细胞HGC2 7,BGC82 3 ,MGC80 3 ,SGC790 1抗脱落凋亡的特性 .为研究肿瘤细胞抗脱落凋亡的机制奠定基础 .方法 :应用软琼脂集落形成实验 ,DNAladder检测 ,流式细胞术等技术观察胃癌细胞脱落培养后细胞生长状况的改变 .结果 :对照组细胞MDCK(狗的正常肾脏上皮细胞系 )在软琼脂中不生长 ,没有形成细胞集落 ;悬浮培养 15h后 ,可见有细胞凋亡特征性的DNAladder出现 ;流式细胞仪检测 ,悬浮培养 2 4 ,4 8h可见有凋亡峰出现 .而HGC2 7等 4种胃癌细胞系在软琼脂中均形成大小不等的细胞集落 ,脱落培养15h后 ,没有细胞凋亡特征性的DNAladder出现 ,流式细胞仪检测 ,与贴壁培养的对照组相比细胞周期没有显著性差异 ,也没有凋亡峰的出现 .结论 :MDCK细胞属于锚着依赖性细胞 ,可出现脱落凋亡 .胃癌细胞HGC2 7,BGC82 3 ,MGC80 3 ,SGC790

AIM: To analyze the characteristics of the anoikis resistance of human gastric cancer cell lines(HGC27, BGC823, MGC803 and SGC7901). METHODS: The growth conditions of the cells were observed by soft agar colony formation assay, DNA laddering assay and flow cytometry assay after the gastric cancer cells were cultured in suspension. RESULTS : The MDCK cells as control group, which were derived from normal canine kidney, did not grow in soft agar and no cell colony was formed. The DNA ladder, the marker of apoptosis, appeared 15 h after the cell culture in suspension and the FCM assay showed that the cells had sub-G1 phase of cell cycle 24 h and 48 h after cell culture in suspension. But in the case of the other four gastric cancer cell lines (HGC27, etc), though different sizes of colon were observed when the cells were cultured in softer agar, there was no DNA ladder 15 h after the cell culture in suspension. By FCM assay, no significant difference was found between the cell cycle of suspensive cells and that of the control cells cultured in adhesion and no sub-G1 phase was observed. CONCLUSION: The MDCK cells are anchorage independent and the phenomena of anoikis can be observed. The four gastric cancer cells, HGC27, BGC823, MGC803 and SGC7901, are generally resistant to anoikis.