MMP-9及TIMP-1在子宫内膜异位症中的表达及意义

Expression and significance of MMP-9 and TIMP-1 in endometriosis

目的 :观察MMP 9和TIMP 1在子宫内膜异位症中的表达 ,明确二者之间的相关性探讨他们在子宫内膜异位症发病机制中的作用 .方法 :利用免疫组化SABC法检测MMP 9和TIMP 1在 84例正常子宫内膜、4 8例内异症的在位内膜、79例内异症的异位内膜组织中的表达 ,检测结果利用SPLM软件进行统计学分析 .结果 :①MMP 9在内异症在位内膜中的阳性表达率为 62 .5 0 % ,内异症异位内膜中阳性表达率为 72 .15% ,与正常内膜组织的表达 (阳性率为 4 0 .4 8% )相比有显著性差异 (P <0 .0 5 ) ;②TIMP 1在异位症在位内膜中的阳性表达率为 70 .83 % ,在异位症异位内膜中阳性表达率为 74 .68% ,与正常内膜组织的表达 (阳性率为 76.19% )相比未见显著性差异 (P >0 .0 5 ) ;③在正常子宫内膜组织中MMP 9与TIMP 1之间具有负向相关关系 ,在异位症的在位内膜组织中以及异位症的异位内膜组织中二者间不具有相关性 .结论 :MMP 9的高表达以及MMP 9/TIMP 1间的比例失调 ,在内异症的病程发展进程中起着重要作用 。

AIM: To observe the expression of matrix metalloproteinase 9 (MMP 9) and tissue inhibitors of matrix metalloproteinase 1 (TIMP 1) in endometriosis, to explore the correlation between the expression of MMP 9 and TIMP 1 in endometriosis and to study the possible role of MMP 9 and TIMP 1 in the pathogenesis of endometriosis. METHODS: Expressions of MMP 9 and TIMP 1 in 84 normal endometrium as well as in 79 ectopic endometrium and 48 eutopic endometrium in endometriosis were detected by immunohistochemistry and the results were analyzed with SPLM statistical software. RESULTS: (1) The expression levels of MMP 9 in ectopic and eutopic endometriotic tissues were significantly higher than those in the normal endometrium (P<0.05). In ectopic and eutopic endometrium of endometriosis and normal endometrium, the positive rates of MMP 9 were 78%, 71% and 50% respectively (P< 0.05 ). (2)In ectopic and eutopic endometrium of endometriosis and normal endometrium, the positive rates of TIMP 1 were 84%, 71% and 80 %, respectively. There were no noticeable difference of the positive expression rates of TIMP 1 in endometriosis and normal endometrium (P> 0.05 ). (3)There was significantly negative correlation between expression of MMP 9 and TIMP 1 in normal endometrium . But there was no correlation between the expression of MMP 9 and TIMP 1 in ectopic/eutopic endometrium of endometriosis. CONCLUSION: The high expression of MMP 9 and the lopsided development on MMP 9/TIMP 1 ratio may participate in the process of invasion and tissue remodeling that is hypothesized to occur in the pathogenesis of endometriosis. They are maybe one of the reasons why ectopic and eutopic endometriatic cells have stronger invasive ability than the normal endometric cells.