益妇宁软胶囊治疗更年期综合征的实验研究

Laboratory Study of the Yi-Fu-Ning Soft Gelatin Capsules in Treating Climacteric Syndrome

目的 :通过观察补肾活血的中药复方益妇宁软胶囊对去势大鼠血清性激素水平、下丘脑单胺类神经递质的影响 ,探讨其治疗更年期综合征的作用机理。方法 :5 0只SD雌性大鼠随机分为 5组 :假手术组、去卵巢对照组、已烯雌酚片组、益妇宁大、小剂量组。给药 4周后用荧光分光光度法测定下丘脑单胺类神经递质 (NE ,DA ,5 HT ,5 HIAA) ;用放射免疫法测定血清性激素水平 ,并测定肾上腺、子宫及胸腺指数。结果 :益妇宁能明显升高大鼠去势后降低的血清E2 水平 (P <0 .0 1) ,提高去势大鼠肾上腺、子宫指数 (P <0 .0 5 ) ;有效降低去势大鼠异常增高的5 HT和 5 HIAA水平 (P <0 .0 1) ,并升高NE和DA的含量 (P <0 .0 1) ,降低 5 HT/NE ,5 HT/DA的比值 (P <0 .0 1)。结论 :益妇宁软胶囊能从多环节调节生殖内分泌功能 ,纠正去势大鼠下丘脑单胺类神经递质紊乱 ,从而达到稳定机体内环境 。

Objective: To study the therapeutic mechanism of YFN on climacteric syndrome by investigating the effect of Yi Fu Ning soft gelatin capsules (YFN),which can nourish the kidney and activate blood,on serum sex hormone levels and hypothalamic monoamine transmitters contents in ovariectomized rats. Method:Fifty female mature Sprague Dawley rats were randomly divided into 5 groups:sham operation;ovariectomy(OVX);OVX with diethylstilbestrol tablets(DT);OVX with YFN(high dose and low dose). After drugs had beengiven for 4 weeks, hypothalamic monoamine transmitters (NE,DA,5 HT,5 HIAA) contents were detected by fluorospectrophotometric method and serum sex hormone by radioimmunoassay. Adrenal gland index, uterus index and thymus index were also determined. Result:YFN could obviously improve serum E 2 and increase adrenal gland and uterus index in OVX rats ( P <0.01 or P <0.05). It could reduce hypothalamic 5 HT and 5 HIAA, which were increased in OVX rats( P <0.01);It could also increase reduced NE and DA in OVX rats and reduce the ratio of 5 HT to NE and 5 HT to DA( P <0.01). Conclusion:YFN can regulate procreate endocrine in various ways and ameliorate the function of hypothalamic monoamine neurotransmitter in OVX rats. So it can stabilize inner condition of body and relieve symptoms of climacteric syndrome.