电针对大鼠脑缺血-再灌注损伤的防护

Protective Effect of Electroacupuncture on Cerebral Ischemia-reperfusion Injury in Rats and Its Mechanism

目的 观察电针治疗缺血性脑病的可能机制。方法 采用结扎大鼠双侧颈总动脉造成的脑缺血 -再灌注损伤模型 ,测定不同时间电针前后海马内 MDA含量和 SOD、GSH -Px活性并计数断头后大鼠喘息次数和持续时间。每日电针“百会”、“风池”、“大钟”及“足三里”穴 30 min共 14 d,取疏 -密波 ,频率 :2 -2 0 H z,强度 :2 .0 A。结果 电针能显著延长大鼠脑缺血 -再灌注后的存活时间 ;降低缺血 -再灌注后大鼠海马的 MDA含量 (d7:2 .5 7± 0 .62 nmol/mg,d14 :1.18± 0 .47nm ol/m g)及提高SOD(d7:5 .18± 1.82 u/m g,d14 :6.91± 2 .47u/mg)及 GSH-Px(d7:9.5 0± 1.0 9u/mg,d14 :11.65± 1.72 u/m g)的活性。结论 电针能提高大鼠抵抗脑缺血 -再灌注的损伤能力 ,其作用机制可能与电针提高动物体内氧化酶活性。

Objective To study the effect of electroacupuncture on cerebral ischemia injury and its mechanism. Methods The rat models were made by ligating the bilateral common carotid arteries. The period and number of gasping after head cutting were recorded. The content of MDA, and the activities of SOD and GSH Px were assayed before and after electroacupuncture. Daily electroacupuncture, with sparse and dense waves at 2 20Hz and 2.0A, was performed at Baihui(GV 14), Fengchi(GB 20), Dazhong(KI 4) and Zusanli(ST 36), 30 minutes each session and 7 or 14 days. Results Electroacupuncture could significantly prolong the surviving period after the head cutting; electroacupuncture could decrease the content of MDA (d7:2.57±0.62 nmol/mg, d14:1.18±0.47 nmol/mg) and increase the activities of SOD (d7:5.18±1.82 u/mg,d14:6.91±2.47u/mg) and GSH Px(d7:9.50±1.09 u/mg,d14:11.65±1.72 u/mg). Conclusion Electroacupuncture can increase the rats' tolerability to cerebral ischemia and anoxia, which may relate to increased activities of oxidase and suppressed production of free radicals.