应用纳络酮治疗细粒棘球蚴致过敏性休克绵羊的肺部形态学观察

Morphological Observation on Lung of Sheep with Anaphylactic Shock Induced by Echinococcus granulosus After Naloxone Treatment

为了观察应用纳络酮治疗细粒棘球蚴致过敏性休克绵羊的肺部形态学变化并探讨机制 ,通过复制感染细粒棘球蚴的绵羊 2 4只 ,用细粒棘球蚴粗制囊液抗原攻击发敏 ,观察并确定休克的发生 ,建立过敏性休克动物模型。攻击发敏后 ,受试绵羊分为 3组 ,每组 8只 ,分别应用纳络酮 ( 组 )、盐酸肾上腺素 ( 组 )治疗 ,生理盐水 ( 组 )作为对照治疗 ,观察6 0 min后处死绵羊 ,测定肺湿 /干重量比值 (W/D) ,观察绵羊肺部形态结构的变化。结果显示 ,两组治疗措施均能明显减轻肺水肿病变 ,而肺部形态学的改善以纳络酮组治疗效果最好 ,肾上腺素组效果次之。

To observe the morphological changes of lung of sheep with anaphylactic shock induced by Echinococcus granulosus (E.g) after naloxone treatment and to approach the mechanism, on the basis of the sheep infected by E.g, 24 sheep were challenged with E.g cystic fluid partially purified antigen 10mL from intrajugular injection to result in anaphylactic shock for establishment of anaphylactic shock model in sheep. Then anaphylactic shock was observed and confirmed after challenged. 24 sheep were divided into naloxone group (group I) ,adrenalin group (groupⅡ) and control group (groupⅢ) at random(n=8). All sheep were sacrificed after observation in 60 minutes,and lung's rate of wet dry weight (W/D) was measured. The morphological changes of lung in sheep were observed. Adrenalin and naloxone were effective in reducing pulmonary edema while control group shown obviously morphological changes. The effect of naloxone in groupⅠ was better than that of adrenalin in groupⅡ. Naloxone is one of the effective drugs for early treatment on anaphylactic shock induced by Echinococcus granulosus.