大黄对大鼠腹腔感染致肺损伤的防治作用

Prophylactic Effects of Rhubarb on Lung Injury Caused by Abdominal Infection in Rats

目的 :探讨大黄对大鼠腹腔感染致肺损伤防治作用的机制。方法 :采用大鼠盲肠结扎并穿孔 (CLP)造成腹腔感染 ,每天在麻醉下经胃管灌注大黄 1次。分别在术后 0 ,2 4 ,48,72 ,96 ,1 2 0h检测肺毛细血管通透性、肺组织的内毒素和肿瘤坏死因子 (TNF α) ,并取支气管肺泡灌洗液 (BALF)进行细胞学分析。结果 :肺毛细血管通透性、BALF的中性粒细胞百分率、肺组织的内毒素和TNF α逐渐增加 ,时间越长越明显 ,但药物组比感染组增加较慢、幅度较小。结论 :大黄可防止内毒素进入肺组织 ,抑制肺内中性粒细胞积聚 ,TNF α释放 ,减轻肺的炎性反应。

Objective: To study the underlying mechanisms of the prophylactic effects in rhubarb on early lung injury caused by the abdominal infection in rats. Method: The cecal ligation and perforation(CLP) was adopt to make a abdominal infection in rats. Rhubarb was injected into the stomaches of rats through tubes once a day. The subgroups of rats were sacrificed at 0, 24, 48, 72, 96 and 120 hours after operation respectively. The permeability of pulmonary capillary, the concentration of endotoxins, and tumor neccrosis factor α(IFN α) in lung were assayed. The cytological analysis for bronchoalvelar lavage fluid(BALF)was perfomed. Result: The permeability of pulmonary capillary, the neutrophil percentage of BALF, and the concentration of endotoxins and TNF α in lung increased gradually, the more time the more obvious, but they were slower and less in drug treated group than in infection group. Conclusion: Rhubarb can significantly mitigate the inflammatory response of lung tissue throuh preventing endotoxins to enter lung and inhibiting accumulation of neutrophils and release of TNF α in lung.