涂片、培养、聚合酶链反应和连接酶链反应几种方法检测宫颈淋球菌感染

The detection of cervical gonococcal infection with LCR PCR methylene blue staining and culture

目的:评价4种方法检测宫颈淋球菌的临床应用价值。方法:用美蓝染色涂片、培养、聚合酶链反应(PCR)淋球菌试剂盒和连接酶链反应(LCR)4种方法对比检测宫颈淋球菌感染情况。结果:4种方法相应的敏感性、特异性、阳性预测值和阴性预测值分别为:涂片方法是56.2%、97.6%、69.2%、95.5%;细菌培养方法是68.8%、100.0%、100.0%、97.1%;PCR方法是:68.8%、97.6%、73.3%、97.0%;LCR方法是:93.8%、92.3%、53.6%、99.4%。如果把涂片和细菌培养并联检测淋球菌,即两者之一阳性即为阳性,则这两种方法并联起来的敏感性为81.3%,特异性为97.6%。结论:涂片和培养并联检测淋球菌比单独涂片和培养的敏感性有所提高,而特异性并没有明显的降低。

Objective: To evaluate the clinical value of four different laboratory tests for cervical gonococcal infection. Methods Abbott LCR kit PCR test kit methylene blue staining of smear and bacteria culture were used to detect gonococcal infection for the cervical samples of 184 females in high risk populations. Results The sensitivity specificity positive predictive value and negative predictive value were 56.2% 97.6% 69.2% and 95.5% respectively by methylene blue staining 68.8% 100% 100% and 97.1% respectively by gonorrhea culture 68.8% 97.6% 73.3% and 97.0% respectively by PCR test kit 93.8% 92.3% 53.6% and 99.4% respectively by LCR kit. The combination of smear and gonorrhea culture showed both of high sensitivity (81.3%) and specificity (97.6%). Conclusions The combination of smear and gonorrhea culture could increase the sensitivity of the smear or culture alone and not decrease the specificity significantly.