侵袭性牙周炎与FMLP受体单核苷酸多态性

The SNP analysis of FMLP receptor in aggressive periodontitis

目的 :研究FMLP受体作为遗传标志与局限型侵袭性牙周炎之间的关系。方法 :提取目标人群 (局限型侵袭性牙周炎L -AgP患者、慢性牙周炎CP患者及正常人群 )的DNA ,PCR扩增后 ,进行DNA基因测序。结果 :在 2 5 9～ 332位点中存在单核苷酸多态 (SNPs) ,L -AgP组与其它两组之间有显著差异。结论 :在汉族人群中 ,L -AgP患者FMLP受体分子细胞外第一环及其邻近的跨膜区氨基酸残基的改变可能是引起患者趋化功能缺陷的分子基础 ,由此导致的受体不能识别细菌趋化物亦可能是引起L

AIM:To evaluate whether FMLP receptor polymorphisms are also associated with localized aggressive periodontitis(L-AgP) in Chinese patients.METHODS:We directly compared DNA encoding the FMLP receptor of three groups(L-AgP patients, CP patients, control group) by means of polymorphisms chain reactions and sequence analysis .RESULTS:DNA sequence of 14 patients indicated single nucleotide polymophisms(SNPs) in FMLP receptor DNA from L-AgP patients when compared to 12 controls( P =0.004526) and 16 CP patients ( P =0.010933) .Single nucleotide base alterations were consistently seen in 259～332.Each alteration resulted in amino acid changes in the first extracellular loop and the third transmembrane of the FMLP receptor molecules. These regions of the FMLP receptor had been shown to play a role in ligand binding and recognize chemotactic peptide. CONCLUSION: This study suggests that a molecular alteration in the first extracellular loop and the third transmembrane of the FMLP receptor molecules in L-AgP patients may play a role in decreased chemotactic activity reported for some L-AgP patients.