不同施药周期DDVP对德国小蠊酶活性的影响

Effects of DDVP at different period on enzyme activity in German cockroaches

目的 探讨德国小蠊在敌敌畏 (DDVP)不同施药周期作用下的酶活性变化规律。方法 采用分光光度法测定德国小蠊乙酰胆碱酯酶、酸性磷酸酯酶、羧酸酯酶及α-乙酸萘脂 (1-NA)和 β -乙酸萘酯 (2 -NA)酶的活性 ,应用SAS软件进行统计分析。结果 随着施药周期的延长 ,乙酰胆碱酯酶酶活性受抑制 ,酶活性降低 ,随后受抑制程度缓慢降低 ,酶活性逐渐上升 ;酸性磷酸酯酶的活性呈上升趋势 ;羧酸酯酶、1-NA和 2 -NA酶都存在随施药周期的延长 ,酶活性上升后又降低的趋势。结论 乙酰胆碱酯酶是DDVP的主要作用靶部位之一 ,受DDVP作用影响最大。酸性磷酸酯酶和 1-NA是DDVP的重要代谢酶。

Objective To investigate the effects on acetylcholine esterase,carboxyesterase,acid-phosphatase,1-NA and 2-NA activity in German cockroaches exposed to DDVP at different period.Methods All enzyme activities were measured by visible spectrophotometry and SAS soft ware was used for statistical analysis.Results With the prolonging exposed to DDVP,the degree of acetylcholine esterase inhibition increase gradually at the beginning and decrease lately.The activity of acid-phosphatase raised generally.The change of carboxyesterase,1-NA and 2-NA activity were in opposition to acetylcholine esterase activity's.Conclusion Acetylcholine esterase was the main target enzyme of DDVP.Acid-phosphatase and 1-NA were the important metabolic enzymes.