mTOR和4E-BP1在结外鼻型NK/T细胞淋巴瘤中的表达及其与预后的关系

Expression of mTOR and 4E-BP1in extranodal Nasal-type NK/T-cell Lymphoma and implication for prognosis

目的:研究哺乳动物雷帕霉素靶蛋白(mTOR)、真核细胞翻译起始因子4E结合蛋白-1(4E-BP1)在结外鼻型NK/T细胞淋巴瘤(ENKTCL)中的表达,及其与患者临床病理因素和预后的关系。方法:采用免疫组织化学染色方法检测36例ENKTCL和20例鼻咽淋巴组织增生患者组织中mTOR和4E-BP1蛋白的表达情况,运用χ~2检验分析其与ENKTCL的临床分期、B症状、一般状况评分及国际预后指数(IPI)之间的关系。结果:ENKTCL组织中mTOR、4E-BP1表达率分别为63.9%和58.3%;鼻咽淋巴组织增生中的mTOR、4E-BP1表达率分别为15.0%和10.0%,2组比较差异均有统计学意义(χ~2=12.355,P=0.0001;χ~2=12.410,P=0.0001),且mTOR、4E-BP1在ENKTCL组织中表达呈正相关(r=0.655,P=0.001)。mTOR与ENKTCL的临床分期有关,且在Ⅲ～Ⅳ期组中的阳性表达率高于Ⅰ～Ⅱ期组,差异有统计学意义(χ~2=17.902,P=0.0001);4E-BP1与ENKTCL的IPI有关,且IPI>2分组的阳性表达率高于IPI≤2分组,差异有统计学意义(χ~2=4.051,P=0.044)。结论:mTOR与下游底物4E-BP1共同作用导致ENKTCL的生长、分化和转移,两者可成为判断ENKTCL预后的有效指标。

Objective:To study the expression of mTOR and 4 E-BP1 in extranodal Nasal-type NK/T-cell Lymphoma(ENKTCL)and the correlation with the clinicopathological factors and prognosis of ENKTCL.Method:Immunohistochemistry was used to detect the expresstion of mTOR and 4 E-BP1 in the tissues of ENKTCL and nasal pharyngeal lymphoid hyperplasia.The relationship between the expression of mTOR and 4 E-BP1 and clinicopathological features was analyzed using the Chi-square test,which including sorting clinical stage,general condition score and international prognostic index(IPI).Result:The expression rates of mTOR and 4 E-BP1 in ENKTCL were 63.9% and 58.3% respectively,and significantly higher than those(15.0% and 10.0%)in patients with nasal pharyngeal lymphoid hyperplasia(χ~2=12.355,P=0.0001;χ~2=12.410,P=0.0001).The positive expression rate of mTOR was linked to clinical staging,and the positive protein expressions in StageⅢ and Ⅳwere higher than those in stageⅠandⅡ(χ~2=17.902,P=0.0001).mTOR expression was related to 4 E-BP1(r=0.655,P=0.001).The positive expression rates of 4 E-BP1 were linked to IPI of lymphoma(χ~2=4.051,P=0.044).Conclusion:High-expressions of mTOR and 4 E-BP1 in patients with ENKTCL are related to the biological progression and recurrence of ENKTCL and are malignant indicators to identify ENKTCL from nasal pharyngeal lymphoid hyperplasia.mTOR and 4 E-BP1 may play important roles to assess ENKTCL prognosis.