OSA患者动脉粥样硬化相关microRNA的表达谱探索分析

Expression profiles of microRNA related to atherosclerosis in patients with OSA

目的:研究OSA患者动脉粥样硬化(AS)相关血清microRNA(miRNA)的表达。方法:筛选116例受试者,包括对照组(n=24)、OSA组(n=32)、AS合并OSA组(n=32)和AS组(n=28)。对受试者进行qRTPCR分析miRNA表达谱。利用Spearman分析相关参数。结果:与对照组相比,OSA组、AS合并OSA组和AS组的miRNA-148a-5p、miRNA-378c、miRNA-127-3p和miRNA-365a-3p表达上调;AS组的miRNA-134-5p表达上调,AS合并OSA组的miRNA-365a-3p高于OSA组。AS相关的miRNA,包括miRNA-148a-5p、miRNA-378c、miRNA-127-3p、miRNA-134-5p和miRNA-365a-3p均与颈动脉内膜中层厚度相关。结论:表达上调的miRNA-148a-5p、miRNA-365a-3p、miRNA-378c和miRNA-127-3p可能是OSA患者发生AS的早期预警标志。

Objective:Recent studies have demonstrated that obstructive sleep apnoea(OSA)may lead to atherosclerosis(AS),but the underlying mechanism remains unclear.MicroRNA(miRNA)may be involved in the gene regulation of AS and also in the occurrence and progression of OSA.The purpose of our study was to investigate the expression of atherosclerosis-related serum miRNAs in OSA patients.Method:We screened 116 participants including normal controls(n=24),OSA alone(n=32),OSA patients with AS(n=32),and patients with AS but without OSA(n=28).qRT-PCR to analyze the microRNA profile was performed in these subjects.Clinical and blood test with lipid parameters were analysed using Spearman correlations.Result:Compared to normal controls,miRNA-148 a-5 p,miRNA-378 c,miRNA-127-3 p and miRNA-365 a-3 p were upregulated in the OSA,OSA-AS,and AS groups;miRNA-134-5 p was only upregulated in the AS group,miRNA-365 a-3 p in the OSA-AS group was higher than that in the OSA alone group.The circulating atherosclerosis-related miRNAs,including miRNA-148 a-5 p,miRNA-378 c,miRNA-127-3 p,miRNA-134-5 p and miRNA-365 a-3 p all correlated with the carotid intima-media thickness.Conclusion:Upregulation of miRNA-148 a-5 p,miRNA-365 a-3 p,miRNA-378 c,miRNA-127-3 p may be an early warning marker of atherosclerosis in OSA patients.