维生素D受体基因单核苷酸多态性与变应性鼻炎易感性相关性分析

Analysis on correlation between single nucleotide polymorphisms of vitamin D receptor gene with susceptibility to allergic rhinitis

目的:探讨维生素D受体(VDR)基因单核苷酸多态性(SNP)与变应性鼻炎(AR)易感性的相关性。方法:选取210例AR患者(AR组)和同期健康体检志愿者180例作为对照组。采集所有受试者空腹静脉血并提取血液DNA,采用聚合酶链式反应-限制性片段长度(PCR-RFLP)法检测VDR基因ApaⅠ(rs7975232)位点、FokⅠ(rs2228570)位点多态性,比较分析不同基因型与AR易感性的关系。Hardy-Weinberg遗传平衡定律验证2组基因型频率是否具有代表性;采用Logistic多元回归法分析影响AR易感性的相关因素。结果:VDR基因rs7975232位点存在3种基因型,分别为野生型纯合子CC、突变杂合子AC、突变纯合子AA;rs2228570位点存在3种基因型,分别为野生型纯合子GG、突变杂合子GA、突变纯合子AA;rs7975232位点、rs2228570位点各基因型分布符合Hardy-Weinberg定律;ApaⅠrs7975232基因型分布比较差异有统计学意义(P<0.05),且AR组C等位基因分布频率显著高于对照组(P<0.05);FokⅠ基因rs2228570位点基因型分布比较差异无统计学意义(P>0.05);Logistic分析显示VDR基因rs7975232位点CC基因型为AR的易感因素。结论:VDR基因rs7975232位点多态性与AR易感性存在一定相关性,CC基因型可能是AR患者的易感因素,而rs2228570位点多态性与AR无明显相关性。

Objective:To investigate the relationship between single nucleotide polymorphism(SNP)of vitamin D receptor(VDR)gene with susceptibility to allergic rhinitis(AR).Method:Two hundred and ten AR patients were selected as AR group,and 180 healthy volunteers from the same period were selected as control group.Fasting venous blood was collected from all subjects and blood DNA was extracted.Polymorphisms at ApaⅠ(rs7975232)and FokⅠ(rs2228570)loci of VDR gene were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP),and the relationships between different genotypes with susceptibility to AR were comparatively analyzed.The Hardy-Weinberg’s law of genetic balance verifies whether the two genotype frequencies were representative;Logistic multiple regression analysis was used to analyze the related factors affecting susceptibility of AR.Result:There were SNPs at rs7975232 locus of VDR gene,which were wild homozygote CC type,mutant heterozygote AC,and mutant homozygote AA.SNPs existed at rs2228570 locus,which were wild homozygote GG,mutant heterozygote GA,and mutant homozygote AA.The distributions of genotypes at rs7975232 and rs2228570 locus of VDR gene conformed to Hardy-Weinberg law.There was a significant difference in the distribution of rs7975232 genotype(P<0.05),and the frequency of C allele in the study group was significantly higher than that in the control group(P<0.05).There was no significant difference in genotype distribution of rs2228570 locus(P>0.05).Logistic analysis showed that CC genotype of ApaⅠwas a risk factor for AR susceptibility.Conclusion:The polymorphism of ApaⅠ(rs7975232)locus of VDR gene is correlated with AR susceptibility.CC genotype may be a susceptible factor for AR patients,but there is no significant correlation between FokⅠpolymorphism and AR.