rmIL-33诱导的nuocyte细胞促进变应性鼻炎小鼠模型的炎症反应

rmIL-33-stimulated nuocytes promote allergic inflammation in mouse model of allergic rhinitis

目的:探讨nuocyte细胞对变应性鼻炎(AR)小鼠模型的作用。方法:将人重组(rm)白细胞介素(IL)-33滴入BALB/c小鼠鼻腔,分离、提纯并体外培养小鼠鼻相关淋巴组织(NALT)中的nuocyte细胞,体外检测该细胞对rmIL-33干预后的反应;用卵清蛋白建立AR小鼠模型,将体外培养的NALT来源的nuocyte细胞过继转移给AR小鼠,检测其变应性炎症反应。结果:rmIL-33干预后小鼠NALT中nuocyte细胞数目明显多于AR组小鼠(t=3.66,P<0.01);体外培养的nuocyte细胞经rmIL-33干预后释放IL-13的浓度显著高于未干预组(t=19.90,P<0.000 1);nuocyte细胞过继转移后,其打喷嚏次数(t=9.89,P<0.000 1)、鼻腔冲洗液中嗜酸粒细胞数目(t=8.17,P<0.000 1)以及IL-13(t=40.47,P<0.000 1)和IL-33的浓度(t=19.89,P<0.000 1)均高于AR小鼠。结论:nuocyte细胞促进了小鼠AR模型的炎症反应。

Objective:The study aimed to investigate the role of nuocytes in allergic rhinitis(AR)murine models.Method:After intranasal administration of recombinant(rm)interleukin(IL)-33 in BALB/c mice,nuocytes were sorted and purified from the mouse nasal-associated lymphoid tissue(NALT).Then,we examined the response of nuocytes to rmIL-33 in vitro.After a murine model of AR was established using ovalbumin,we adoptively transferred the cultured NALT-derived nuocytes to mice models,and determined allergic responses in them.Result:rmIL-33 expanded nuocytes in NALT of mice compared with AR mice(t=3.66,P<0.01),and increased production of IL-13 from these cells in vitro in comparison with unstimulated nuocytes(t=19.90,P<0.000 1).After adoptive transfer of nuocytes,sneezing(t=9.89,P<0.000 1),numbers of eosinophils(t=8.17,P<0.0001),concentrations of IL-13(t=40.47,P<0.000 1)and IL-33(t=19.89,P<0.000 1)in nasal lavage fluid were all enhanced when compared with AR mice.Conclusion:Nuocytes promote allergic inflammation in a murine model of AR.