牙槽神经离断、磨牙结扎及龈沟液注入联合使用建立牙周炎小鼠模型的研究

The model of periodontitis was established by the combined use of alveolar nerve dissection,molar ligation and gingival crevicular fluid injection

目的:探索建立典型牙周炎实验性小鼠模型的方法。方法:选取54只健康小鼠,随机分为对照组(小鼠第一磨牙和第二磨牙间隙注射生理盐水)、条件对照组(结扎丝压迫牙龈,同时离断磨牙下方牙槽的神经)、实验组(结扎丝压迫牙龈,同时离断磨牙下方牙槽的神经,配合牙周炎患者龈沟液的注入)三组,每组均18只小鼠。比较三组小鼠饲喂6周后牙龈指数、牙齿松动度构成的差异,比较三组小鼠研究前后血清中Ig G、IL-4、IL-6含量的统计学差异,记录牙龈局部临床特征、形态学和病理学检查结果。结果:三组小鼠牙龈指数差异具有统计学意义(P<0. 05),来源于实验组和对照组、实验组和条件对照组之间的差异;三组小鼠牙齿松动度差异具有统计学意义(P<0. 05),来源于实验组和对照组之间的差异。研究后三组小鼠血清中Ig G、IL-4、IL-6含量的差异均显著(P<0. 05),两两比较各组之间的差异均显著(P<0. 05)。实验组小鼠牙龈红肿和溃疡明显、牙周探针探入容易出血,小鼠平均嵴顶在釉牙骨质界下约1. 2 mm;病理结果显示,有大量的破骨细胞破坏了牙槽骨和牙槽嵴顶结构,同时组织内有浆细胞浸润。结论:小鼠牙龈局部离断牙槽神经,对磨牙进行结扎,第一磨牙和第二磨牙间隙注入牙周炎患者的龈沟液,有助于建立典型的牙周炎小鼠模型。

Objective:To explore the method of establishing the mouse model for the experimental periodontitis,and to provide reference for the basic research.Methods:Fifty-four healthy mice were randomly divided into three groups that each group of 18 mice,blank group(the first molar and second molar clearance in mice injected saline),conditions,the control group(ligation silk oppression gums,at the same time from the inferior alveolar nerve broken grinding)and the experimental group(ligation silk oppression gums,from the inferior alveolar nerve broken grinding at the same time,cooperated with periodontitis patients with gingival sulcus liquid injection).The gingival index and tooth loosening composition,the serum Ig G,IL-4 and IL-6 contents and the local clinical features,morphology and pathological examination results of the gingiva of the three groups were recorded after six weeks feeding.Results:The difference in gingival index composition between the three groups was significant(P<0.05).The three groups showed significant difference in the composition of tooth looseness(P<0.05),which was derived from the difference between the experimental group and the blank group.After the study,Ig G,IL-4,IL-6 contents in serum of the three groups were significantly different(P<0.05),and the differences between the two groups were significant(P<0.05).In the experimental group,the mice showed obvious gingival redness and ulcer,and periodontal probe was prone to bleeding.The average crest of the mouse was about 1.2 mm below the bone boundary.Pathological results indicated that a large number of osteoclasts damaged the structure of alveolar bone and alveolar crest,and there was plasma cell infiltration in the colleague tissue.Conclusion:The gingival space of the first molar and the second molar was injected into the gingival crevicular fluid of the patients with periodontitis,which was helpful to establish a typical model of periodontitis mice.