双亚苄基哌啶与蛋白酶体抑制剂对舌鳞癌细胞存活率和周期的影响

Effect of Bis-Benzylidine Piperidon and proteasome inhibitor on the viability and cycle process of tongue squamous cancer cells

目的:探讨双亚苄基哌啶(Bis-Benzylidine Piperidon,RA190)与蛋白酶体抑制剂MG132对舌鳞癌细胞活率和周期的影响。方法:分别培养舌鳞癌细胞CAL27、TCA8113。设无细胞空白组、无加药对照组和不同终浓度(0. 1,0. 2,0. 4,0. 5,0. 8,1. 6,3. 2,6. 4μg/m L) RA190或MG132实验组,培养24 h后,CCK8法酶标仪检测各组细胞存活率,流式细胞仪分析各组细胞周期分布情况。结果:相同浓度下,MG132组舌鳞癌细胞存活率低于RA190组(P<0. 05);MG132与RA190组CAL27细胞存活率均低于TCA8113细胞(P<0. 05);MG132组的CAL27细胞G2/M期数量占比高于对照组,差异均有统计学意义(P<0. 05)。结论:舌鳞癌细胞对MG132作用的敏感性高于RA190;CAL27细胞对MG132、RA190的敏感性高于TCA8113;MG132阻滞CAL27细胞于G2/M期,可能提高了CAL27细胞对MG132敏感性。

Objective:To investigate the effects of Bis-Benzylidine Piperidon(RA190)and proteasome inhibitor MG132 on the viability and cycle of tongue cancer cells.Methods:The cultured tongue cancer cells CAL27 and TCA8113,were divided into Blank and control group(no drug addition)and experimental group,respectively.The experimental group was treated with different concentrations(0.1,0.2,0.4,0.5,0.8,1.6,3.2,6.4μg/m L)of RA190 or MG132 for 24 hours.The cell survival rate of each group was measured by CCK8 through enzyme labeling instrument,and the cell cycle distribution of each group was analyzed by flow cytometry.Results:The survival rate of CAL27 cells treated with MG132 or RA190 was lower than that of TCA8113 cells.The survival of CAL27 and TCA8113 cells treated with MG132 was lower than that with RA190.The proportion of CAL27 cells in G2/M phase treated with MG132 was higher than that in control group.All of the difference was statistically significant(P<0.05).Conclusion:The sensitivity of tongue cancer cells to MG132 is higher than that of RA190.The sensitivity of CAL27 cells to MG132 or RA190 is higher than that of TCA8113 cells.MG132 blocks CAL27 cells in G2/M phase,and it may increase the sensitivity of CAL27 cells to MG132.