转录因子FOXO1在前列腺癌组织中的表达及临床意义

Expression and clinical significance of transcription factor FOXO1 in prostate cancer tissues

目的:检测前列腺癌(PCa)组织与良性前列腺增生(BPH)组织中转录因子FOXO1的表达情况,探讨FOXO1与PCa临床病理参数之间的关系。方法:收集2010年1月～2016年12月我院53例PCa患者行根治性前列腺切除术后病理标本和经尿道前列腺电切术的53例BPH病理标本,采用组织芯片方式收集组织标本,使用免疫组化染色法检测FOXO1表达情况,分析FOXO1表达同PCa患者临床病理参数之间的关系。结果:BPH组织中FOXO1呈高表达,PCa组织中FOXO1表达显著低于BPH组织(39.6%vs.86.8%,P<0.05),中低危PCa组织的相对表达量显著高于高危PCa组织(P<0.05)。FOXO1表达与PCa PSA水平、病理T分期、淋巴结转移、Gleason评分显著相关(P<0.05),Gleason评分越高FOXO1水平越低,PCa组织中FOXO1表达与患者年龄、前列腺体积、切缘阳性无明显相关性(P>0.05)。结论:FOXO1在PCa组织中异常低表达,与PCa侵袭转移相关,FOXO1表达与PCa PSA水平、病理T分期、淋巴结转移和Gleason评分密切相关。

Objective: To detect the expression of FOXO1 in prostate cancer(PCa) and benign prostatic hyperplasia(BPH) tissues, and to investigate the relationship between FOXO1 and clinicopathological parameters in PCa. Method: Fifty-three cases of PCa pathology specimens and 53 cases of BPH pathology specimens after TURP from January 2010 to December 2016 in our hospital were collected. Tissue samples were collected by tissue microarray and immunohistochemical staining was used to detect the expression of FOXO1. The relationship between FOXO1 expression and clinicopathological parameters in patients with PCa was analyzed. Result: FOXO1 was highly expressed in BPH tissues, while FOXO1 expression in PCa tissues was significantly lower than that in BPH tissues(39.6% vs. 86.8%, P<0.05). The expression of FOXO1 in low-middle-risk PCa tissues was significantly higher than that in high-risk PCa tissues(P<0.05). FOXO1 expression was significantly correlated with PSA level, pathological T stage, lymph node metastasis and Gleason score in PCa(P<0.05). The higher the Gleason score was, the lower the FOXO1 level was. However, the FOXO1 expression in PCa wasn’t correlated with age, prostate volume or pathological positive margin(P>0.05). Conclusion: FOXO1 is abnormally low expressed in PCa tissues, which is correlated with the invasion and metastasis of PCa. FOXO1 expression is closely related to PSA level, pathological T stage, lymph node metastasis and Gleason score in PCa.