免疫组化在鉴别皮脂腺瘤和基底细胞癌中的应用

Application of immunohistochemical examination in differentiation of sebaceoma and basal cell carcinoma

目的:探讨免疫组化在鉴别皮脂腺瘤和基底细胞癌(BCC)中的意义。方法:对31例皮脂腺瘤和31例结节型BCC患者标本分别行常规苏木精-伊红(HE)染色和免疫组化染色,并观察其结果。结果:免疫组化示31例皮脂腺瘤患者上皮抗原(EA)均为阴性;上皮膜抗原(EMA)在导管及成熟皮脂腺细胞中均呈阳性表达;24例雄激素受体(AR)呈阳性表达,余7例均为阴性;D2-40阳性表达28例,阴性3例。而结节型BCC患者中30例EA阳性,1例阴性;EMA阳性4例,阴性27例;AR阳性表达15例,阴性16例;D2-40 5例灶状阳性,余26例均阴性。结论:免疫组化示皮脂腺瘤患者EA阳性、EMA阴性和D2-40阳性,可较好地与结节型BCC相鉴别。

Objective:To investigate the significance of immunohistochemical examination in differentiating sebaceoma from basal cell carcinoma(BCC). Methods:The samples from 31 cases of sebaceoma and 31 cases of nodular BCC were assessed with H&E staining and immunohistochemical staining. Results:Immunohistochemical examination showed negative for epithelial antigen(EA) in all cases of sebaceoma, and positive in 30 cases of nodular BCC. Epithelial membrane antigen(EMA) were positive in sebaceous duct and mature sebaceous gland cells of sebaceoma, while only 4 cases were positive in nodular BCC.Androgen receptor(AR) was positive in 24 cases of sebaceoma and in 15 cases of nodular BCC. Positive staining of D2-40 was observed in 28 cases of sebaceoma and 5 cases of nodular BCC. Conclusions:The results of immunohistochemistry suggest that the positive rates of staining for EA and D2-40 along with the negative rates of EMA can differentiate sebaceous adenoma from nodular BCC.