藏药“佐太”长期给药对大鼠肾脏潜在氧化损伤的初步研究

Study on potential oxidative damage of long-term drug application of Tibetan Medicine “Zuotai” on rats kidney

目的:初步探讨长期给予藏药"佐太"对大鼠肾脏可能存在的氧化损伤,以期揭示藏药"佐太"长期用药对肾脏可能存在的过氧化毒性作用,为"佐太"制剂在临床上的安全合理使用提供一定的理论依据。方法:将120只SD大鼠随机分为空白对照组和藏药"佐太"高、中、低剂量组,通过对大鼠给予"佐太"混悬液90天、180天及停药恢复30天,对动物进行一般观察,检测大鼠肾脏中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,计算其肾脏系数并进行肾组织病理学检查。结果:连续给药"佐太"90天、180天及停药30天后,与空白对照组比较,藏药"佐太"各给药组大鼠外观体征、行为活动、进食及排便情况均无明显异常,各给药组大鼠肾脏中MDA含量、SOD活性、肾脏系数及肾组织病理学与空白对照组比较均无明显差异(P>0.05)。结论:推测长期使用藏药"佐太"对大鼠肾脏不产生明显的氧化损伤和肾脏毒性作用,但其安全性问题仍然需要广泛而深入地研究。

Objective: To preliminarily discuss the possible oxidative damage of long-term drug application of Tibetan medicine 'Zuotai' on rats kidney in order to reveal the potential peroxidation and toxicity of 'Zuotai' during the period of longterm use and provide certain theoretical basis for safty and rational use of 'Zuotai' preparation in clinic. Method: 120 SD rats were randomly divided into normal group and Tibetan medicine 'zuotai' groups involving high, middle and low dose. The general daily signs were observed, contents of malondialdehyde(MDA), activity of superoxide dismutase(SOD), and kidney indexes were detected, renal histopathological examination was carried out at 90 d and 180 d respectively after drug application and at 30 d after drug withdrawal. Result: No obvious abnormalities at 90 d and 180 d after continuous drug application and at 30 d after drug withdrawal was found in 'Zuotai' group when compared with normal group. And there was no obvious difference in MDA content, SOD content, kidney indexes and renal histopathology between normal group and Tibetan medicine 'Zuotai' group(P>0.05).Conclusion: It is speculated that long-term application of Tibetan medicine 'Zuotai' has no obvious oxidative damage on kidney of rats and renal toxicity, but its security still needs extensive and in-depth research.