聚合酶链技术用于艾滋病毒检测的文献评价

Assessment of literatures for detection of human immune-deficiency virus by PCR technique

目的 客观评价PCR技术在人类免疫缺陷病毒(human immune-deficiency virus,HIV)检测中的性能,了解聚合酶链技术(polymerase chain reaction,PCR)PCR技术作为艾滋病筛查方法的临床应用价值。方法 以diagnosis Tests(诊断性试验),AIDS(艾滋病),PCR(聚合酶链反应),HIV(人类免疫缺陷病毒)为检索词,对1991年～2001年的MEDLINE和CBM进行了回顾性机检,并根据国际公认标准对纳入文献进行分析。结果 共检索到567篇论著性文章,纳入53篇。其中与金标准进行对比的有47％,同时计算了和能计算出敏感度、特异度、预测值等统计学指标的文章数量分别为25％、23％和23％。没有文章计算似然比。结论 PCR技术在HIV检测特别是筛查中的应用还处在探索阶段,有待于提高研究方案的总体设计水平。

Objectives To analyze and assess the status of detection of human immune-deficiency virus (HIV) by PCR , and to find a new screening test of HIV.Methods Using the following keywords 'diagnosis tests' , 'AIDS', 'PCR' and 'HIV',we searched the Medline and CBM from 1991 to 2001.Then we assess each of diagnosis test according to the international standards. Results 567 articles were searched, in which 53 articles were chosen to assess. In these 53 articles , it was found that 47% applied comparison with Golden Standard, 25% calculated sensitivity, 23% calculated specificity, and 23% calculated predictive value, no likelihood ratio was calculated in these articles. Conclusions It was still a kind of pilot-study to apply PCR to screening detection of HIV.The design methods of study should be improved.