幽门螺杆菌感染患儿血清IL-6、IL-8、IL-10水平变化

Clinical significance on the changes of serum IL-6,IL-8and IL-10levels in children with H.pylori infection

为探讨各种细胞因子在幽门螺杆菌相关性胃肠粘膜病中的作用机理及意义 ,采用ELISA法 ,检测35例幽门螺杆菌阳性患儿血清IL_6、IL_8、IL_10水平 ,并与幽门螺杆菌阴性组31例作对照。结果显示两组间IL_6、IL_8分布水平差异有极显著性 (P<0.001) ;两组间IL_10分布水平差异有显著性 (P<0.05)。幽门螺杆菌阳性组与阴性组IL_6平均秩和之差为16.38,均数分别为108.46pg/ml及51.32pg/ml;IL_8平均秩和之差为34 ,均数分别为163.09pg/ml及92.36pg/ml;IL_10平均秩和之差为 -3.32 ,均数分别为12.56pg/ml及15.88pg/ml。幽门螺杆菌阳性组血清IL_6与IL_8间呈正相关 (r=0.349 ,P<0.001) ;IL_10与IL_6、IL_8间无明显相关性。提示细胞因子IL_6、IL_8、IL_10均参与了幽门螺杆菌感染后的致病过程 ;幽门螺杆菌感染胃肠粘膜产生的炎症反应损伤与IL_6、IL_8的过量产生有关 ,血清IL_10对炎症有抑制作用 。

To explore the role of the various cytokines,such as IL_6,IL_8and IL_10in the pathogenesis of the patients with gastrointestinal mucosa lesions related to H.pylori infection and their significance,the serum levels of IL_6,IL_8and IL_10were assayed with ELISA in35H.pylori positive children with31H.pylori negative children as the control group.The results indicated that the difference of the serum IL_6.IL_8level distribution between these two groups had great significance(P<0.001).Whereas the difference of the serum IL_10level distribution between these two groups also had marked significance(P<0.005).The deviation of serum IL_6levels in average rank sum test between H.pylori positive and H.pylori negative group was16.38.The mean values were108.46pg/ml and51.32%respectively.The deviation of serum IL_8level in average rank sum test was34.The mean values were163.09pg/ml and92.36pg/ml seperately.The deviation of serum IL_10level in average rank sum test was _3.32with the mean value equal to12.56pg/ml and15.88pg/ml.The serum IL_6level had a positive correlation with the IL_8level(r=0.349,P<0.001).But,the serum IL_10level had no relationship to the IL_6and IL_8level indicating that cytokines IL_6,IL_8and IL_10might play a role in the pathogenesis of the patients with H.pylori infection.So the inflammatory response and lesions in the gastrointestinal mucosa happened in the H.pylori infection had some relationship to the overdue production of the IL_6and IL_8.While the serum IL_10could inhibit the inˉflammatory response thus providing the theoretical basis for clinical diagnosis and treatment of the H.pylori infection asˉsociated disorders.