祛风通络方药对急性缺血性中风内皮素及其基因表达的影响

EFFECT OF QFTL TCP ON ENDOTHELIN AND GENE EXPRESSION IN ACUTE CEREBRAL ISCHEMIA

目的 :观察祛风通络方药治疗急性缺血性中风的疗效及对血浆内皮素含量与内皮素mRNA表达的影响。方法 :选择 6 0例发病在 4 8h以内的急性缺血性中风患者 ,观察用药前后血浆内皮素及神经功能缺损积分的变化。实验研究探讨祛风通络方药对MCAO大鼠 12 ,2 4 ,36和 4 8h各时间点神经功能缺损、梗死体积、内皮素含量、内皮素基因表达的影响。结果 :祛风通络方药组患者治疗后神经功能缺损积分及血浆内皮素 - 1含量较对照组显著降低 ,P <0 .0 5。MCAO大鼠 12 ,2 4 ,36和 4 8h神经功能缺损积分、脑梗死体积、海马内皮素 - 1含量及内皮素 - 1mRNA表达等与假手术组比较有显著差异 ,P <0 .0 5。祛风通络方药组与MCAO组比较 ,上述各项指标均有显著改善 ,P <0 .0 5。结论 :祛风通络方药能有效改善急性脑缺血损伤神经功能缺损积分与内皮素 - 1含量及基因表达。

Objective:To observe the effect of QFTL TCP on mRNA expression of endothelin in plasma and the quantity of endothelin.Method:We select 60 acute cerebral ischemia patients occurred in 48h, be observed on the change of endothelin in plasma and Neurological Deficit Score. Our research work is on the effect of QFTL TCP, time is 12h,24h,36h,48h item including the Neurological Deficit Score, ischemia range, quantity of gene expression of endothelin.Results:The Neurological Deficit Score and endothelin declined in QFTL group( P <0.05).There is distinct difference between MCAO and SHAM in Neurological Deficit Score, range of cerebral ischemia, quantity of endothelin and mRNA expression in hippocampi, P <0.05.There is distinct difference in QFTL group compared with MCAO, P <0.05.Conclusion:QFTL TCP can improve Neurological Deficit Score and gene expression of endothelin.