谷胱甘肽对大鼠肺纤维化模型的干预作用

Interfering Effect of Glutathione on Pulmonary Fibrosis in Rats

目的 观察谷胱甘肽对博莱霉素 (Bleomycin ,BLMA5)致大鼠肺间质纤维化模型的干预作用。方法 大鼠随机分为对照组、BLM模型组、谷胱甘肽组。按BLM 5mg·kg-1体重复制大鼠肺间质纤维化模型 ,2 4h后谷胱甘肽组每天腹腔内注射谷胱甘肽注射液 12 0mg·kg-1。于 7、14、2 8d观察各组肺间质纤维化形成的病理变化和肺匀浆中谷胱甘肽 (GSH)及胶原的含量变化。结果 与模型组比较 ,谷胱甘肽组病理变化与模型组一致 ,但较BLM模型组减轻 ;肺组织匀浆GSH含量明显增高 (P <0 0 1) ,胶原含量无明显变化 (P >0 0 5 )。结论 腹腔内注射谷胱甘肽具有对抗氧自由基、保护肺脏免受氧化损伤的作用 。

Objective To observe the effect of glutathione on bleomycin-induced pulmonary fibrosis in rats .Methods Fifty-four SD rats in good conditions were divided into three groups at random:control group ,BLM group,glutathione(GSH) group.On experimental day(day 0),the rats were intratracheally instilled with bleomycin(5mg·kg -1 .body weight),and then treated with intraperitoneal injection of glutathione .On days 7,14,28 after instillation ,six rats of each group were sacrified respectively and the lungs were harvested for histopathological examination and determinations of GSH and hydroxyproline(HYP).Results Pathological findings of GSH group were the same as BLM group,but compared with BLM group,pathomorphologic changes in GSH group was a small extent.The content of GSH in lung tissue was increased significantly(P<0 01),whereas the content of HYP was nearly the same as the BLM group satistically(P>0 05).Conclusions Glutathione has anti-oxidation and may protect lung tissue free from oxidation injury, but it has no protecting effect on collagen deposition caused by bleomycin.