慢性阻塞性肺疾病患者T淋巴细胞活化与凋亡的变化及临床意义

Changes of Activation and Apoptosis of T-lymphocyte in Peripheral Blood of Patients With COPD and Their Clinical Significance

目的 探讨慢性阻塞性肺疾病 (COPD)急性加重期和稳定期患者T淋巴细胞活化与凋亡的变化及临床意义。方法 将 64例COPD患者分成 2组 :急性加重期组 ( 3 4例 )和稳定期组 ( 3 0例 ) ,各组用荧光单抗CD3 + 、CD4+ 、CD2 5+ 、CD95+ 、CD3 + /CD2 5+ 、CD8+ /CD2 8+ 、CD8+ /CD2 8-对淋巴细胞进行标记 ,用流式细胞仪计数。结果 与COPD稳定期相比 ,急性加重期患者体内CD3 + 、CD95+ 、CD3 + /CD2 5+ 、CD8+ /CD2 8+ T淋巴细胞减少 ,CD4+ 、CD2 5+ T淋巴细胞无明显变化 ,CD8+ /CD2 8-T淋巴细胞增加。结论 COPD急性加重期患者T淋巴细胞总数减少 ,亚群发生变化 ,免疫调节功能紊乱 ,故在治疗疾病过程中 ,除对症处理外 ,必须配合使用调节细胞免疫药物 ,以改善免疫功能。

Objective To investigate the changes of activation and apoptosis of T-lymphocyte in peripheral blood of patients with chronic obstructive pulmonary disease(COPD) at the attack and relieved stage and their clinical significance. Methods 64 cases of COPD were divided into 2 groups: the acute stage(n=34)and the relieved stage groups(n=30). The T-lymphocytes were marked by fluoromonoantibodies CD 3 +, CD 4 +, CD 25 + , CD 95 +, CD 3 +/CD 25 +, CD 8 +/CD 28 +,CD 8 +/CD 28 - and was counted by flow cytometry . Results Compared with the relieved stage, in patients with COPD at the acute attack period,the CD 3 +, CD 95 +, CD 3 +/ CD 25 +, CD 8 +/ CD 28 - were increased, CD 4 + ,CD 25 + had not obvious change, but CD 8 +/ CD 28 + was increased. Conclusions In COPD at the acute attack stage, the immune function was disordered as the result of the change of T subtype of lymphocytes and the reduction of total T lymphocyte.So except for treatment in accordance with their symptoms,immunodrug of cells should be used in order to raise the immune function of the patients.