钙在玉米愈伤组织继代及盐诱导脯氨酸积累中的作用

Roles of calcium in the subculture of embryogenic callus and proline accumulation induced by salt in maize

通过筛选试验 ,将N6 培养基Ca2 + 含量从原来的 1 13mmol/L提高到 5mmol/L ,对玉米幼胚胚性愈伤组织的继代具有明显促进作用 ,在一次继代过程中 ,Ca2 + 含量 5mmol/L的培养基培养的胚性愈伤组织相对生长量比Ca2 + 含量 1 13mmol/L的原培养基增加 0 8倍。将愈伤组织接种于含NaCl的N6 继代培养基上 ,其生长极显著受抑制 ,脯氨酸积累增加。培养基中同时添加 5mmol/LCa2 + ,不仅在一定程度上缓解盐分对生长的抑制作用 ,而且能明显促进游离脯氨酸的积累。

The selection experiment showed that modified N6 medium by increasing Ca 2+ content from 1 .13mmol/L to 5 mmol/L promoted obviously embryogenic callus subculture grown from maize immature embryos.During one time of subculture,the growth rate of embryogenic callus of subcultured on the medium containing 5 mmol/L of Ca 2+ increased by 0 .8 time compared with that subcultured on the medium contenting 1 .13 mmol/L of Ca 2+ .Addition of NaC1 to the N 6 subculture medium with 10g/L,the growth of embryogenic callus was inhibited significantly,and proline content increased .At the same time,addition of 5 mmol/L Ca 2+ to the N 6 subculture medium with 10g/L NaCl,the inhibition by salt was not only alleviated,but also the proline accumulation was promoted .