基层医院凡士林纱布高压灭菌失败原因分析

High Pressure Sterilization of Vaseline Gauze in Primary Hospital: Analysis of Failure Causes

目的 观察凡士林纱布厚度、消毒灭菌次数及在锅中各位置的灭菌效果 ,对凡士林纱布的灭菌失败原因进行分析。方法 将嗜热脂肪杆菌芽胞 ATCC795 3布片 ,夹置于各种厚度凡士林纱布的中心放于铝储物盒中 ,经手提式压力蒸汽灭菌器灭菌后做细菌定性分析。结果 经 2次高压灭菌的 32层以内的凡士林纱布中的嗜热脂肪杆菌芽胞 (ATCC795 3)全部杀灭 ,杀灭指数为 5× 10 5～ 5× 10 6。结论 采用手提式压力蒸汽灭菌器灭菌方法可靠 ,灭菌失败原因与凡士林纱布厚度、消毒灭菌次数及在锅中各位置有关 ,应控制凡士林纱布的厚度 ,不易过厚(不超过 32层 ) ,并经 2次高压消毒灭菌 ,置于锅中上层为消毒效果最佳位置。

OBJECTIVE To observe the different sterilization effect by different thickness of the gauze, different sterilization times and the location in the pot, and analyse the failure causes in sterilizing vaseline gauze. METHODS Put Bacillus stearoqlthermophilus gemma ATCC7953 in a slide, and put it in vaseline gauze, which is located in aluminium storage box. After steam sterilization in portable pressurized sterilizer, the qualitative analysis was done. RESULTS The test monitoring was done by several times. The result was that the gemma of ATCC7953 contaminated in the gauze in layer 32 was killed completely after 2 times of high pressure sterilization. The killing index was 5×10 5 5×10 6. CONCLUSIONS Portable steam sterilizer is reliable in sterilization, and the failure for sterilization is influenced by thickness of the gauze, times for sterilization and the location in the pot. The thickness of the gauge shall be limited (no more thicker than 32 layers), high pressure sterilization shall be done twice, and the location of upper level in the pot is the best position for the sterilization.