姜黄素对心脏压力过负荷大鼠主动脉细胞外基质的影响

Effects of curcumin on the extracellular matrix of aorta in cardiac overloaded rats

目的 :探讨姜黄素对心脏压力过负荷大鼠主动脉细胞外基质的影响。方法 :腹主动脉部分结扎致大鼠心脏压力负荷增高 ,姜黄素灌胃 3wk后测定大鼠单位长度主动脉湿重 体重比值 ;Woessner法测主动脉胶原含量 ,酶谱半定量测定法测基质金属蛋白酶 (MMP2 )活性。结果 :模型组主动脉湿重 体重比值增加、组织胶原含量增高、主动脉MMP2 活性下降。姜黄素灌胃 3wk后降低主动脉湿重 体重比值 ,主动脉胶原含量降低 ,MMP2 活性改变不明显。结论 :姜黄素可逆转心脏压力过负荷大鼠主动脉的肥厚 ,其机制主要与抑制胶原合成有关。

AIM: To study the effect of curcumin on the extracellular matrix of aorta in cardiac overloaded rats. METHODS: Cardiac overloaded model was made by partial click of the abdominal aorta in Sprague Dawley rats. Curcmin, 100 mg·kg -1 ·d -1 for 3 weeks, was given by po 1 week after the click operation. The ratio of aorta wet weight to body weight was calculated; the collagen content was measured with Woessner's method and the activity of matrix metallproteinase 2 was analyzed with zymography. RESULTS: In cardiac overloaded rats, the wet weight and collagen content of aorta was increased, and the activity of matrix metallproteinse 2 of aorta was decreased. Compared with the cardiac overloaded rats without any drugs, the increased weight and collagen content of aorta was reversed, but the activity of matrix metallproteinse 2 of aorta was seldom changed in curcumin treated rats. CONCLUSION: Curcumin can reverse the hypertrophy of aorta of cardiac overloaded rats mainly by means of inhibiting the synthesis of collagen.