肺癌中P^(53)基因DNA水平突变与核蛋白的水平突变比较

Comparison between P^(53) Gene DNA Mutation and Neucleoprotein Mutation in Lung Cancer

目的 研究肺癌 P53基因 DNA水平和蛋白水平的突变。方法 应用 PCR- SSCP银染技术检测了 5 4例肺癌标本。结果 2 2例 DNA水平突变阳性 ,突变率为 41%。 32例在核蛋白水平突变 ,突变率 5 9%。其中 2 2例 DNA突变阳性者 ,核蛋白突变均阳性。而 32例 DNA水平突变阴性者 ,核蛋白突变阳性者 9例 ,占 2 8%。P53 DNA突变与核蛋白的突变之间有显著相关性 ,并且两者都与患者的淋巴结转移有关 (P<0 .0 1)。结论 PCR- SSCP是一种高灵敏快速检测 P53是否存在异常的有效方法。但是 ,P53突变阳性患者预后较差。

Objective To investigate the comparison between P 53 gene mutation and neucleoprotein mutation in lung cancer.Methods 54 samples of lung cancers were collected and PCR SSCP technique was used to detect the mutation.Results There were 22 DNA mutations (41%) and 32 neucleoprotein mutations (59%).In 22 DNA mutations,neucleoprotein mutations were also found,while in 32 no DNA mutations,9 neucleoprotein mutations were found(28%).P 53 gene DNA mutation showed significant correlation with neucleoprotein mutation and both correlated with lymph node metastasis(P<0.01).Conclusion PCR SSCP was a highly sensitive method to detect if P 53 gene mutated.Patients with P 53 mutations suffered poor prognosis.