nm23-H1基因在大肠癌组织中表达的临床意义

Clinical Significance of nm23-H1 Gene Expression in Colorectal Carcinoma

目的 探讨 nm2 3- H1基因表达与大肠癌生物学行为及预后的关系。方法 应用抗 nm2 3- H1单克隆抗体 ,采用免疫组化 ABC方法对 12 1例大肠癌组织进行标记分析。结果 nm2 3- H1蛋白在大肠癌低分化组的阳性率明显低于高、中分化组 (P<0 .0 5 ) ;淋巴结转移组的阳性率明显低于淋巴结未转移组 (P<0 .0 5 ) ;而临床 Dukes分期中 A期组阳性率分别显著高于 C期组及 D期组 (P<0 .0 5 )。结论 nm2 3- H1表达缺失的大肠癌具有更强的浸润及淋巴结转移能力 ,nm2 3- H1基因可作为判断大肠癌预后的一个重要指标。

Objective To investigate the relationship between the expression of nm23-H1 gene and biological behavior of colorectal carcinoma and its prognosis.Methods nm23-H1 gene protein was detected in 121 colorectal carcinoma tissues with immunohistochemical ABC method.Results The positive rate of nm23-H1 in poor differentiated colorectal carcinomas was significantly lower than that in high differentiated one(P<0.05),and the positive rate in group with lymph node metastasis was much lower than that in group without lymph node metastasis (P<0.05).In clinical stage,the positive rate of Dukes A stage group was obviously higher than that of Dukes C and D stage group,respectively(P<0.05).Conclusion Colorectal carcinoma with the loss of nm23-H1 gene expression had a stronger ability to infiltration and lymph node metastasis,and nm23-H1 gene could be used as an important marker for the assessment of prognosis of colorectal carcinoma.