中药宝宝乐对幼龄厌食大鼠胃泌素及CCK的影响

Effects of Baobaole on gastrin and CCK in anorexia juvenile rats

目的 :研究运脾平肝复方宝宝乐口服液对小儿厌食症动物模型胃泌素及胆囊收缩素 (cholecystokinin ,CCK)的调节作用 .方法 :模拟小儿厌食症的主要病因制作幼龄厌食大鼠模型 ,用宝宝乐口服液治疗 ,用放免检测技术测定实验动物下丘脑、胃窦部及外周血胃泌素和CCK的含量 ,并与单纯运脾复方儿宝颗粒对照 .结果 :模型大鼠胃泌素含量胃窦部减少(P <0 .0 5 )、血清无明显变化、下丘脑未测出 ,下丘脑和血浆CCK 8浓度均明显增高 (131.12± 2 8.5 6vs 91.0 8± 18.6 8,P <0 .0 5 ;4 86 .95± 113.32vs 2 6 2 .15± 95 .0 9,P <0 .0 1) ;宝宝乐和儿宝颗粒治疗组胃窦部胃泌素含量、下丘脑和血浆CCK 8浓度均恢复正常 ,宝宝乐治疗组下丘脑CCK 8浓度回降较儿宝颗粒明显 (85 .6 7± 2 2 .6 2vs10 8.36± 2 0 .97,P <0 .0 5 ) .结论 :宝宝乐能调节该模型中枢及外周胃泌素和CCK的分泌与释放 .

AIM: To study the regulatory effects of Baobao le on gastric antrum and cholecystokinin (CCK) in anorexia model of juvenile rats. METHODS: The rat anorexia model, made by simulating the mechnism of children anorexia, was treated with Baobaole and the levels of gastric antrum and CCK in hypothalamous, antrum pyloricm, and plasma of the model were examined by radioimmunoassay (RIA) and compared with those of the group treated by Erbao Granules. RESULTS: The results showed that the gastric antrum level of model rats decreased in antrum pylorium ( P <0.05) , but no change was observed in plasma. The CCK level in the hypothalamus and plasma of model rats showed a significant increase ( P<0.05, P < 0.01) . The gastrin and CCK levels of the two groups treated by Baobaole and Erbao Granules were normal but the Baobaole group made a more significant decrease in hypothalamic CCK level than Erbao group ( P <0.05). CONCLUSION: Baobaole can regulate the secretion and release of gastrin and CCK in central and peripheral areas and may play an important role in inhibiting the secretion and release of CCK in our model hypothalamus.