中药安迪粉针剂对小鼠巨噬细胞cAMP含量的影响

Effects of Traditional Chinese Medicine Andi on cAMP in murine peritoneal macrophages

目的 :观察中药安迪粉针剂 (Andi)对小鼠巨噬细胞(MФ)cAMP含量的影响 .方法 :采用细胞培养和竞争性蛋白结合分析法测定小鼠腹腔MФ中cAMP含量 .结果 :①cAMP水平的变化具有规律性 :VB在 3min以内可引起小鼠腹腔MФ中cAMP浓度快速升高 ,6min时达到峰值 ,之后缓慢下降 ,至30min时基本恢复原来水平 .RG和Andi则是 3min开始升高 ,到 15min达峰值 ,然后逐渐下降 ,180min时已基本恢复至原来水平 ;其升高程度显著高于VB(P <0 .0 5 ) ;②Andi能明显引起小鼠腹腔MФcAMP升高 ,并在 (6～ 4 8)g·L-1剂量范围时呈量效依赖关系 .结论 :Andi引起小鼠MФcAMP浓度升高 。

AIM: To observe the effects of traditional Chinese medicine Andi on cAMP in murine peritoneal macrophages (MΦ). METHODS: cAMP concentration in macrophages of mice abdominal cavity was determined by using cell culture and competitive protein conjunction analysis. RESULTS: ① cAMP level had some regular changes. Within 3 min following ip. Venenum Bufonis (VB), the cAMP concentration rose rapidly and reached the peak value at 6 min, then declined gradually and recovered to the primary level in 30 min. At 3 min following the administration of Radix Ginseng (RG) and Andi, the cAMP concentration rose rapidly, reached the peak value at 15 min, then declined gradually and recovered to the primary level in 180 min. cAMP of RG and Andi reached a higher level than that of VB ( P <0.05 or P <0.01); ② Andi obviously increased the cAMP concentration in murine peritoneal macrophages in a dose and effect dependent manner at 6～48 g·L -1 . CONCLUSION: Andi can increase the cAMP concentration in murine peritoneal macrophages, which may partly accounts for the mechanism of Andi in improving the mice immune functions.