摘要
目的:为加速体外培养的人鼻中隔软骨细胞的增殖,观察类胰岛素样生长因子I(IGF-I)对体外培养软骨细胞的影响。方法:体外培养人鼻中隔软骨细胞,MTT法观察IGF-I对软骨细胞增殖的影响;流式细胞仪检测IGF-I对软骨细胞周期的影响;斑点杂交法了解IGF-I对软骨细胞II型胶原mRAN的影响。结果:IGF-I浓度大于5ng/ml即可促进体外培养软骨细胞的增殖,缩短软骨细胞增殖周期,有助于II型胶原mRNA的合成。结论:IGF-I促进人鼻中隔软骨细胞增殖与缩短细胞周期有关,并可能通过促进软骨细胞II型胶原mRNA的合成使软骨细胞保持表型稳定。
AIM:To investigate the effects of IGF-I on the human nasal septal chondrocyte s cultured in vitro for their rapided growth.METHODS:The nasal septal chondrocyt es were cultured in vitro, the growth of chondrocytes was detected by MTT. We us ed the flow cytometer to study the cell cycle of the chondrocytes effected by IG F-I, and the collagen II mRNA was studied by dot hybridization also.RESULTS:IGF -I can significantly increase the proliferation of chondrocytes when its concen tration over 5 ng/ml, it can shorten cell cycle of chondrocytes cultured in vitr o, and improve the synthesis of the collagen II mRNA.CONCLUSION:The growth of hu man nasal septal chondrocytes effected by IGF-I was related to the shortening o f cell cycle. IGF-I may enhance the output of collagen II mRNA to maintain the chondrocytes phenotype.
出处
《中国临床康复》
CSCD
2003年第4期554-555,共2页
Chinese Journal of Clinical Rehabilitation