高氧液治疗油酸型肺损伤家兔的实验研究

Therapeutic effect of hyperoxia solution on oleic acid induced acute lung injury in rabbits

目的:研究高氧液(hyperoxiasolution,HO)对油酸型急性肺损伤(acutelunginjury,ALI)家兔的治疗作用。方法:以林格氏液为基液制备HO。将18只家兔随机分为油酸组(A组),高氧液组(B组)和正常对照组(C组),每组6只。A、B各组均按文献方法经耳缘静脉注入油酸0.06mL/kg建立ALI模型,C组动物注入等量生理盐水。B组于注射油酸30min时经右侧颈外静脉给予HO20ml/Kg,A、C组给予等量林格氏液。各组分别在ALI模型制备前(0min)、注射油酸后30,60,120min时间点经右颈外动脉进行血气分析,并测定肺水含量,肺体比值及病检。结果:与C组相比A组PaO2、PaCO2显著降低(P<0.01),肺水含量及肺体比值显著增高(P<0.01),镜下见肺灶性出血,间质、肺泡水肿,大量炎细胞浸润。B组经HO治疗后迅速提升PaO2、PaCO2(P<0.05),减轻肺水肿(P<0.01),大部分肺组织无明显病理学改变。结论:HO对油酸型ALI家兔有治疗作用。

AIM:To investigate the effect of hyperoxia solution (HO) on acute lung injury in rabbits induced by oleic acid.METHODS:Eighteen rabbits were randomly divided into three groups of 6 each: oleic acid group (A group), hyperoxia solution grou p(B group) and control group(C group).At different time points blood gas analysi s and content of lung water were also observed.RESULTS:Compared with that in the control group, PaO2,PaCO2 decreased(P< 0.01), content of lung water markedl y increased in A group(P< 0.01). After treatment with HO, PaO2,PaCO2 signifi cantly increased(P< 0.05),content of lung water decreased(P< 0.01).CONCLUSION :Hyperoxia solution has beneficial effect on ALI induced by oleic acid.