Dynamic expression of several grow factors in process of osteoblasts grafts to promote healing of osteoporotic fracuture

AIM:To study the biological function of transforming growth factor a1(TGF-a 1),vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF) during the process of osteoblasts allografts to accelerate fracture healing of osteoporosis rats.METHODS: Set up the fracture models of osteoporosis rats and do osteoblasts grafts; during different periods in the process of fracture heal ing by using immunnohistochemistry to detect the expression of TGF-a1,VEGF,bFG F and in situ hybridization to study the expression of bFGFmRNA,VEGFmRNA,TGF-a 1 mRNA,also using image analysis to deal with it.RESULTS:In experimental group, Osteoblasts can survive, even proliferate in fracture areas;VEGF,bFGF could be s een positive after 7 days of after osteoblast grafts,and there have the highest quantities about 14 days of post-transplantation.TGF-a1 could be seen positiv e after 3 days of post-transplantation,and there have the highest quantities ab out 7-10 days of post-transplantation.However there are not obviously high qua ntities in control.CONCLUSION:Osteoblasts grafts enhance bone fracture healing of osteoporosis rats.TGF-a1, VEGF and bFGF play very important roles in accel erating fracture healing of osteoporosis rats.