中国两种地方猪种内源性逆转录病毒的亚型分析

Analysis of Subtype of Porcine Endogenous Retrovirus in Two Species of Chinese Pigs

目的 对两种中国地方猪种 (五指山猪和版纳微型猪近交系 )的 86头个体进行猪内源性逆转录病毒(PERV)亚型分析。方法 取引物 Env A、Env B和 Env C分别用于鉴定 PERV的 A、B和 C亚型 ,并对 86头个体进行PCR法分析。结果 在所有被检测个体中 ,77.9%的个体的基因组中同时有 PERV的 A、B两种亚型 ,另外 2 2 .1%的个体的基因组中则只有 PERV的 A或 B亚型 ;所有个体的外周血白细胞中均未检测到 PERV的 C亚型。结论 PERV在中国五指山猪和版纳微型猪近交系的外周血白细胞中只有 A、B两种亚型 ,并且以

Objective To analyze the subtype of porcine endogenous retrovirus (PERV) in two species of Chinese pigs Wu zhishan pig and Banna minipig inbred; the total number of pigs being eighty six. Methods Three primers which are specific for the three PERV subtypes (subtypes A,B and C) were used to amplify the genome DNA extracted from the pigs. Results In all tested pigs' genome, subtype C was not found; in 77.9% pigs, double positive envAB were found; in 22.1% pigs, only A or B was found. Conclusion In the genome of peripheral leukocytes from Wuzhishan pig and Banna minipig inbred, only subtypes A and B exist, and envAB predominate.