猪皮组织胶原原纤维的三维结构

Three-dimensional Structure of Collagen Fibril of Pigskin

目的 研究猪皮组织胶原原纤维的三维结构 ,为猪皮组织作为组织工程材料提供基础数据。方法 应用扫描探针显微镜和透射电镜对猪皮组织胶原原纤维进行观察。结果 原纤维的表面存在着周期约为 6 7nm凹凸结构 ,原纤维的直径 5 7～ 135 nm,在猪皮组织的乳头层、网状层和下网状层间存在很大差异 ;观察到的原纤维的端头为膨大的球形 ,原纤维的长度为 5～ 13μm ,原纤维之间的侧向聚集与其周期性横纹结构相吻合并在其首尾间存在搭接。结论 获得了猪皮组织胶原原纤维三维结构的数据 。

Objective To probe deply into the three dimensional structure of pigskin collagen fibril and provide the basic data for using the pigskin tissue as tissue engineering material.Methods Scanning Probe Microscope (SPM) and Transmission Electron Microscope (TEM) were employed to study the three dimensional structure of collagen fibril in pigskin tissue. Results Microscopy revealed that pigskin collagen fibril had periodic transverse groove (i. e. D periodicity) which was about 67 nm. The diameter of fibril ranged from 57 to 135 nm, showing much difference among the papillary layer, reticular layer and infra reticular layer. The length of fibril varied from 5 to 13 μm. Both ends of fibril were rotund and slightly bulgy. The fibrils assembled and the D periodicities were homologous in flank. In axial direction fibrils were found staggered end by end.Conclusion The data obtained in this study on the three dimensional structure of pigskin collagen fibril are of significance to researches in tissue engineering materials.