微卫星DNA序列在人-鼠脐血移植模型中的应用

Application of Short Tandem Repeat DNA in Model of Human-mouse Cord Blood Transplantation

目的 评价微卫星 DNA序列在人 -鼠脐血异种移植中的应用价值。方法 采用 PCR扩增短串联重复序列 (PCR- STR)技术及克隆形成细胞体外培养方法 ,观察移植后受鼠体内的造血细胞植入情况、造血生长因子对其的影响。结果 1静脉输注 1～ 5× 10 7个 MNC/只鼠可使受鼠体内表达供者 DNA,而经腹腔注射不能建立人 -鼠移植模型。 2人脐血细胞移植 SCID小鼠可以重建小鼠造血功能 ,其表达水平与外源细胞因子的使用无关。结论STR- PCR分析可以从分子水平对移植物植入状态进行微量和准确的鉴定 ,该方法为人 -鼠脐血移植模型的鉴定提供了简便。

Objective To evaluate the significance of applying short tandem repeat DNA in the model of human mouse cord blood transplantation. Methods The techniques of STR PCR and colony forming cell culture were used to examine the engrafting and influence of hematopoietic growth factor on process in the mice after transplantation of human cord blood(CB). Results ①Human DNA expression was detected in the mice that had received tail vein injection of human CB(1 5×10 7 MNC per mouse) but it was not detected in the mice that have received intraperitoneal injection of human CB. ②Human CB transplanted in SCID mice could reconstitute the hematopoietic function of the mice; the expression level of human cell was not related with the use of hematopoietic growth factor. Conclusion STR PCR analysis provide a simple and accurate indication of engraftment in the model of human mouse cord blood transplantation.