面神经再生微环境的蛋白质分布及针刺对其的影响

Using Two-dimensional Gel Electrophoresis to Analyze the Regeneration Microenvironment of Facial Nerve and Its Response to Acupuncture

目的 探讨面神经再生微环境的蛋白质分布及穴位针刺对该微环境的影响。方法 建立兔面神经再生微环境动物模型 ,采用蛋白质双向凝胶电泳技术对面神经再生微环境中的蛋白质进行了检测和分析。结果 神经再生液中的蛋白质主要分布于相对分子质量 80× 10 3以下 ,等电点 4～ 7之间 ,对照组检测到 80 0± 5 3个蛋白质点 ,针刺组检测到 85 0± 78个蛋白质点 ,在匹配的点中 ,有十几种蛋白质发生了明显变化。

Objective To investigate the distribution of proteins in the regeneration microenvironment of facial nerve and its response to acupuncture. Methods We created an animal model using rabbit to imitate the regeneration microenvironment of facial nerve and analyzed the proteins in the microenvironment through the two dimensional gel electrophoresis. Results We found that most proteins in the regeneration fluid were under relative molecular mass 80×10 3 and in the range of pI 4 7. An average of 800±53 protein spots was found in the control group compared to the 850±78 spots in the acupuncture group. In the matching spots, more than ten proteins were different between the two groups. Conclusion There is no evidence to show that acupuncture has a positive effect on the regeneration microenvironment of facial nerve. Facial nerve regeneration Proteome