壬基酚对成年雄性SD大鼠生殖功能的影响

Adverse Effects of Nonylphenol on the Reproductive Function of Adult Male SD Rats

目的 研究壬基酚对 SD雄性大鼠生殖功能的影响。方法 将成年雄性 SD大鼠经口染毒壬基酚(5 0、10 0、2 0 0 mg/ kg) 2 8d后 ,处死 ,测定生殖功能相关指标 ,并作睾丸组织病理学检查。取成年雄性大鼠睾丸细胞进行体外培养 ,壬基酚染毒 ,测定睾丸酮分泌量的变化 ,并对其间质细胞、支持细胞和生精细胞的超微结构进行电镜观察。结果 经口染毒壬基酚剂量≥ 10 0 m g/ kg可引起大鼠精子计数和活力的显著降低 ,睾丸组织病理学切片显示曲细精管萎缩 ,生精能力下降。睾丸细胞体外培养研究显示 ,壬基酚能抑制睾酮的分泌 ,且电镜观察可见间质细胞内质网肿胀 ,表明合成功能减弱。

Objective To assess the adverse effects of both in vivo and in vitro exposure to nonylphenol on the reproductive function of adult male SD rats. Methods Adult male SD rats were sacrificed after they had received orall nonylphenol at 50, 100, 200 mg/kg for 28 days. Both indications related to their reproductive function and histopathological section of testis were analyzed. In vitro study of testicular cells from adult male rats by use of culture solution with different nonylphenol concentrations was conducted. Testosterone production in cell culture medium was examined and the ultrastructure of Leydig cell, Sertoli cell and spermatogenesis cell were observed under electron microscope. Results In vivo study of the experiment animals in comparison with the controls revealed that sperm count and motility in testis of adult rats exposed to nonylphenol at doses of 100 mg/kg and above were significantly decreased, and the histopathological sections of testis showed atrophied seminiferous tubules and decreased spermatogenesis. In vitro study of testicular cell culture showed that testosterone secretion was inhibited. And electron microscopy disclosed that the endoplasmic reticulum of Leydig cell swelled, which might indicate the reduction of testosterone biosynthesis. Conclusion Nonylphenol at higher dose directly impairs the reproductive function of adult male SD rats.