生长抑素对急性胰腺炎大鼠血中IL-10和TGF-β_1的影响及意义

Effect of Somatostatin on Modulation of IL-10 and TGF-β_1 During Acute Pancreatitis

目的 观察生长抑素对急性胰腺炎时血中抗炎性细胞因子 IL- 10及 TGF- β1 的影响并探讨其意义。方法 实验用雄性 SD大鼠分为正常对照组 (n=6 )、胰腺炎组 (n=8)和生长抑素组 (n=6 ) 3组。胰腺炎组采用开腹胰胆管注射 5 %牛黄胆酸钠 0 .6 m l/ kg制成胰腺炎模型 ;生长抑素组于胰腺炎诱导成功后 ,皮下注射生长抑素 3μg/ (kg· h) ,12 h重复一次。分别于 2 h、6 h、2 4 h处死动物 ,抽血查 IL- 10和 TGF- β1 ,同时检测胰淀粉酶和胰腺湿重 ,血白细胞记数。结果 在急性胰腺炎后 2、6 h IL- 10及 TGF- β1 无明显变化 ,但在急性胰腺炎后 2 4 h血中的 IL-10及 TGF- β1 则显著增高 (P<0 .0 5 ) ;生长抑素组上述细胞因子较胰腺炎组明显下降 (P<0 .0 5 )。 3组动物 2 4 h血淀粉酶分别为 374 .2± 92 .84 U/ L,16 82 .2 5± 4 5 9.35 U/ L,14 97.4 0± 2 2 5 .9U/ L,胰腺湿重分别为 0 .5 3± 0 .0 9g,1.5 3± 0 .13g,1.6 8± 0 .15 g。对比生长抑素治疗前后发现 ,反映胰腺炎病情严重性的胰腺湿重和淀粉酶两项指标均无明显好转 (P>0 .0 5 )。结论 生长抑素在急性胰腺炎后期抑制抗炎性细胞因子 IL- 10及 TGF- β1

Objective To observe the effect of somatostatin on modulation of IL 10 and TGF β 1 in acute pancreatitis and investigate the mechanism. Methods SD male rats were divided into 3 groups: Group 1, the normal rats as control( n =6); Group 2, the rats with acute pancreatitis induced by ductual injection with 5% sodium cholate sulfur at the volume of 0.6 ml/kg without any other treatment( n =8); Group 3, after pancreatitis were induced, the rats were injected hypodermically with somatostatin 3 μg/(kg·hr)( n =6).The animals were killed at 2,6 and 24 hours after operation (each period n =7). The blood samples were taken for measurement of IL 10 and TGF β 1 (by ELISA). The weight of pancreatic tissue and amylopsin was also observed. Results Both IL 10 and TGF β 1 in blood of acute pancreatitis increased significantly ( P <0.05).After the injection of somatostatin, IL 10 and TGF β 1 were found remarkably decreased at 24 hours postoperation ( P <0.05). No changes of the weight of pancreatic tissue and amylopsin were observed after the injection of somatostatin. Conclusion Somatostatin can depress the increase of inflammatory associated cytokines (IL 10 and TGF β 1) that increased remarkably in acute pancreatitis, which is one of the most important reasons why somatostatin cannot relieve pancreatitis.